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Cat.No. : CSC-RO1044 Host Cell: Panc-1
| Cat. No. | CSC-RO1044 |
| Description | Panc-1-CLDN18-A2 cell line is engineered to stably overexpress human CLDN18 isoform 2 (CLDN18.2). |
| Gene | CLDN18-A2 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | Panc-1 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Cryopreservation conditions: Liquid nitrogen. Cryopreservative: 55% basic medium + 40% FBS + 5% DMSO.
A: Under normal culture conditions, passaged 2-3 times per week.
A: Human CLDN18-A2 Stable Cell Line-Panc-1, although derived from human pancreatic cancer cells, is adherent.
A: BSL-1
A: Human CLDN18-A2 Stable Cell Line-Panc-1 is epithelial-like.
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