ISRE-Luc/EF1α-mCherry Reporter Cell Line - PANC-1

The ISRE-Luc/EF1α-mCherry Reporter Cell Line - PANC-1 is a dual-reporter human pancreatic carcinoma cell line specifically developed for monitoring the Interferon-Stimulated Response Element (ISRE) signaling pathway. It stably integrates the firefly luciferase gene driven by a promoter containing multiple tandem ISRE repeats, enabling quantitative and sensitive measurement of type I and type III interferon-induced transcriptional activation. Concurrently, the cell line constitutively expresses mCherry red fluorescent protein under the control of the strong EF1α promoter, serving as an internal control for cell viability, signal normalization, and direct visual monitoring. Established from the human PANC-1 line, this model delivers high signal-to-noise performance for studying innate immune signaling in pancreatic cancer cells.

This specialized cell line is ideal for high-throughput screening of immunomodulatory compounds, small molecules, and biological agents that activate or inhibit the JAK-STAT/ISRE signaling cascade. Researchers utilize it to measure cell-based interferon activity, assess host antiviral signaling responses, and analyze tumor cell reactivity to cytokine therapies. By normalizing inducible firefly luciferase luminescence against baseline mCherry fluorescence, accurate evaluation of pathway activity is maintained while controlling for cell density variation or compound toxicity. Furthermore, the constitutive mCherry reporter facilitates real-time cell tracking, live-cell imaging, and flow cytometry assays, making it a valuable tool for oncology, immunology, and targeted drug discovery workflows.