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Normalized cDNA Library Construction Service

Creative Biogene is a leading biotechnology company which can provide high quality normalized cDNA library construction services for customers worldwide. Creative Biogene’s special normalization procedures and highly experienced staffs are able to decrease the prevalence of high abundance transcripts and equalize transcript concentrations in a cDNA sample. Besides, with years of experience in the cDNA library construction field, Creative Biogene can provide the most affordable normalized cDNA library construction services with fastest turnaround time to satisfy your downstream needs.

Normalized cDNA libraries are produced using a special normalization procedure which can enhance the gene discovery rate of a cDNA library and facilitate the identification and analysis of rare transcripts. This procedure is imperative for transcriptome sequencing, and useful in other applications, such as functional screening, construction of specific RNA libraries, and transcript end sequence profiling. As a leading supplier in cDNA library construction services, Creative Biogene can provide you with high-quality normalized cDNA library construction services to ensure your satisfaction in a timely and professional manner.

Applications:

  1. Discovery rare genes.
  2. Transcriptome sequencing

Features:

  1. High normalization degree.
  2. Low vector background and high percentage of recombinants.
  3. The libraries can be made directionally and in the vector of your choice.
  4. Competitive prices.
  5. Fast turnaround time.

Creative Biogene offers subtractive cDNA library construction services for your scientific research as follows:

  1. Extraction RNA from a variety of samples.
  2. Synthesis full-length cDNA and normalization.
  3. Directional cloning the target cDNAs into any vector.
  4. Production of a library with at least 106 cfu.
  5. Verification of library quality by randomly picking clones and determining insert sizes by restriction digest or DNA sequencing.

References:

  1. Zhulidov PA, Bogdanova EA, Shcheglov AS, et al. A method for the preparation of normalized cDNA libraries enriched with full-length sequences. Russian Journal of Bioorganic Chemistry, 2005, 31(2): 170-177.
  2. Zhulidov P A, Bogdanova E A, Shcheglov A S, et al. Simple cDNA normalization using kamchatka crab duplex - specific nuclease. Nucleic acids research, 2004, 32(3): e37-e37.

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