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Yeast Two-hybrid cDNA Library Construction Service

Creative Biogene is a leading biotechnology company which can provide high quality yeast two-hybrid cDNA library construction services for customers worldwide. Creative Biogene’s advanced technologies and highly experienced staffs are able to assist you in all aspects of total RNA extraction, reverse transcription, library construction and qualification. With years of experience in the cDNA library construction field, Creative Biogene can provide you with high-quality yeast two-hybrid cDNA library construction services to ensure your satisfaction in a timely and professional manner.

Yeast two-hybrid cDNA library is a powerful molecular biology tool in studying the protein interactions, to find the domain that plays a key role in protein-protein interaction or to discover a new protein interaction with target proteins. The establishment of the yeast two-hybrid library is based on the well understanding of the regulation of the transcription initiation process of eukaryotic cells. As a professional supplier in cDNA library construction services, Creative Biogene can provide you with the most affordable yeast two-hybrid cDNA library construction services with fastest turnaround time to satisfy your downstream needs.

Applications:

  1. Investigation interactions between new proteins and target protein.
  2. Identification the protein cascade substrates.
  3. Investigation the effects of mutation on protein binding.

Features:

  1. High transformation efficiency.
  2. Low false positive rate.
  3. Competitive prices.
  4. Fast turnaround time.

Creative Biogene offers yeast two-hybrid cDNA library construction services for your scientific research as follows:

  1. Extraction RNA from a variety of samples.
  2. Synthesis ds cDNAs.
  3. Cloning the target cDNAs into AD plasmid.
  4. Production of a library with at least 106 cfu.
  5. Verification of library quality by randomly picking clones and determining insert sizes by restriction digest or DNA sequencing.

References:

  1. Young KH. Yeast two-hybrid: so many interactions, (in) so little time. Biology of reproduction, 1998, 58(2): 302-311.
  2. Joung JK, Ramm EI, Pabo CO. A bacterial two-hybrid selection system for studying protein–DNA and protein–protein interactions. Proceedings of the National Academy of Sciences, 2000, 97(13): 7382-7387.
  3. Hurt JA, Thibodeau SA, Hirsh AS, et al. Highly specific zinc finger proteins obtained by directed domain shuffling and cell-based selection. Proceedings of the National Academy of Sciences, 2003, 100(21): 12271-12276.

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