Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RO01308
Host Cell : HT1080 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RO01308 |
| Description | This cell line is engineered to stably overexpress human fibroblast activation protein alpha (FAP). |
| Product Type | Human gene overexpression stable cell line |
| Target Gene | FAP |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HT1080 |
| Host Cell Species | Homo sapiens (Human) |
| Applications |
1) investigation of gene function 2) screening and validation of antibodies, agonists, antagonists 3) anticancer drug development |
| Size | One vial of frozen cells, typically >1x10^6cells/vial |
| Stability | This cell line is stable at least 10 passages. |
| Quality Control |
1) Real-time qRCR analysis of gene mRNA overexpression level 2) Flow cytometry analysis of gene surface overexpression level 2) mycoplasma detection |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Target Gene | FAP |
| Background | The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014] |
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Our lab focuses on targeting Fibroblast Activation Protein (FAP), and this HT1080 stable line is fantastic. The FAP expression is intensely high and stable, making it the perfect target cell line for our CAR-T cell efficacy and cytotoxicity assays. A truly exceptional research tool.
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