Cat.No. : CSC-RO0700 Host Cell: MC38
| Cat. No. | CSC-RO0700 |
| Description | This cell line is engineered to stably overexpress mouse Fap in MC38 which is a murine colon adenocarcinoma cell line and is commonly used in the field of oncology. |
| Gene | Fap |
| Gene Species | Mus musculus (Mouse) |
| Host Cell | MC38 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Developing efficient therapies in the framework of cancer therapy depends on an awareness of the mechanisms of immune evasion by tumors. With an eye on boosting the immune response against diseases including melanoma and colorectal cancer, researchers have investigated creative methods to replicate viral infections in tumor cells. New research showed that pMHCI-IgGs, which show viral peptides on the tumor surface, may induce a strong CD8 T cell response, therefore reducing minor tumor metastases. However, larger solid tumors, such as those from MC38 cells, remained resistant despite significant immune activation. This highlights the complexity of tumor microenvironments and the need for advanced strategies in immunotherapy.
Figure 1. The researchers employed pMHCI-IgG and TCB fusion proteins to activate CD8 T cells, assessing their efficacy against MC38-FAP tumor cells through flow cytometry and cell lysis assays. (Fischer C, et al., 2020)
Creative Biogene's Mouse Fap Stable Cell Line - MC38 offers a valuable tool for researchers investigating the interplay between immune responses and tumor biology. Our MC38 cell line is engineered to express mouse fibroblast activation protein (FAP), enabling the study of FAP-targeting therapies and their effects on immune modulation.
A: The Fap gene encodes for the fibroblast activation protein, which is a serine protease. This protein is primarily expressed in fibroblasts and plays a crucial role in the remodeling of extracellular matrices during tissue repair and wound healing.
A: The fibroblast activation protein has been found to contribute to cancer progression by promoting tumor growth, invasion, and angiogenesis. It can also enhance the degradation of extracellular matrices, facilitating the spread of cancer cells to other parts of the body.
A: Yes, targeting the Fap gene and its protein products has potential therapeutic significance in cancer treatment. Inhibiting fibroblast activation proteins can inhibit tumor growth, invasion, and metastasis, thereby slowing down cancer progression. In addition, the Fap gene can also serve as a biomarker for cancer diagnosis and prognosis.
A: The expression of the Fap gene is regulated by various factors, including growth factors, cytokines, and extracellular matrix components. In normal conditions, Fap expression is typically low, but it can be upregulated in response to tissue injury or inflammation. In pathological conditions such as cancer, the expression of Fap gene can be dysregulated, contributing to tumor progression and metastasis.
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The Mouse Fap Stable Cell Line - MC38 offers customized services, allowing researchers to tailor the cell line to their specific experimental requirements. This flexibility is particularly beneficial for studies that involve unique genetic modifications or specific research questions related to the Fap gene.
The Mouse Fap Stable Cell Line - MC38 undergoes stringent quality control measures to ensure the reliability and consistency of the product. Rigorous testing and validation processes, including genetic stability and functional assays, guarantee that the cell line meets the highest industry standards, providing researchers with confidence in their experimental results.
The Mouse Fap Stable Cell Line - MC38 is delivered using a secure and efficient system that preserves the viability and genetic integrity of the cells. The supplier's attention to detail in shipping and handling ensures that the cell line arrives in optimal condition, ready for immediate use in research applications, thereby facilitating seamless integration into research workflows.
The Mouse Fap Stable Cell Line - MC38 is shipped and stored under optimal conditions, ensuring the maintenance of cell viability and genetic stability. The supplier's careful attention to shipping logistics guarantees that the cell line arrives in peak condition, ready for immediate use in research applications.
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