Mouse Fap Stable Cell Line - CT26

Name: Mouse Fap Stable Cell Line - CT26
SKU: CSC-RO0690
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0690

Host Cell : CT26 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Documents

Cat. No.	CSC-RO0690
Description	This cell line is engineered to stably overexpress mouse fibroblast activation protein (Fap) in CT26.
Target Gene	Fap
Gene Species	Mus musculus (Mouse)
Host Cell	CT26
Host Cell Species	Mus musculus (Mouse)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Fap fibroblast activation protein [ Mus musculus ]
Gene Symbol	Fap
Gene Description	fibroblast activation protein
GeneID	14089
Uni ProtID	A2AS87
mRNA Refseq	NM_007986.2
Protein Refseq	NP_032012.1
Chromosome Location	2 C1.3; 2 36.0 cM
Function	endopeptidase activity; hydrolase activity; peptidase activity; peptidase activity; protease binding; serine-type endopeptidase activity; serine-type peptidase activity;

CSC-RO0690-Mouse Fap Stable Cell Line - CT26-Datasheet

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Background

Case Study

Applications

Q & A

Customer Reviews

The Mouse Fap stable cell line CT26 is a genetically modified cell line designed to stably express Fibroblast Activation Protein (Fap), a cell surface glycoprotein associated with tumor growth and progression. The Fap gene, which codes for Fap, is expressed in stromal cells across a range of organs and is involved in tissue repair and extracellular matrix remodeling.

Derived from the CT26 mouse colon cancer cell line, this stable cell line is an invaluable resource for researching the relationship between tumor cells and the surrounding environment. Under typical culture conditions, CT26 cells can develop in monolayers because they have an epithelial shape and stick to culture surfaces.

The researchers used genetically encoded FAPdL5**, targeted to various subcellular locations, to bind a heavy atom-substituted dye (MG2I-ester), forming an on-demand TAP-producing singlet oxygen. Stable HEK-293 cell lines expressing FAP in distinct compartments were generated, selected by FACS for equal expression, and verified by fluorescence microscopy. Photocytotoxicity was assessed using MTS assays and cell death was characterized by flow cytometry post-illumination. FAP locations included cytoplasm, ER lumen, membrane, mitochondria, and nucleus.

Figure 1 shows that the sensitivity of HEK cells and CT26 cells with mitochondrial localization of FAP to singlet oxygen is significantly different. (doi: 10.3389/fchem.2020.592941.) Figure 1. HEK cells and CT26 cells with mitochondrial localization of FAP have significantly different sensitivities to singlet oxygen generation. CT26 cells show significant resistance to singlet oxygen generated by mitochondria. (Liang P, et al., 2020)

Using Creative Biogene's Mouse Fap Stable Cell Line - CT26 allows improved experiments to study the role of FAP in tumor cells with a CT26 cell line that more closely approximates cancer biology. This improvement improves the stability and consistency of experiments and helps to better understand the potential of FAP in cancer treatment.

1. Tumor Microenvironment Studies: Examines the relationships that exist between stromal cells and tumor cells, with a particular emphasis on the function of Fibroblast Activation Protein (Fap) in the development and spread of tumors.

2. Cancer Therapy Research: Using the expression of Fap in CT26 cells, this study assesses possible treatments that target Fap to stop the growth of tumors.

3. Extracellular Matrix Remodeling: Examines Fap's role in extracellular matrix remodeling and tissue repair, which is crucial to comprehending cancer metastasis.

4. Drug Screening: Offers a means of identifying anti-cancer medications that might impact pathways connected to Fap, thereby offering new perspectives on treatment approaches.

5. Tumor-Stroma Interaction: Focuses on the ways in which tumor behavior is influenced by stromal cells expressing Fap, which can help develop treatments that target the tumor microenvironment.

Customer Q&As

How stable is Mouse Fap expression? Is there detailed stability experimental data?

A: Mouse Fap Stable Cell Line-CT26 has been tested for multiple passages and Fap expression remains stable. We conducted experiments with multiple consecutive passages and verified the expression level of Fap through qPCR and Western Blot. The results showed that there was no significant change in the expression of Fap.

Has this cell line been functionally validated? Has it been tested in in vivo or in vitro experiments?

A: This cell line has been functionally verified in vitro, proving that the Mouse Fap it expresses has biological activity. We confirmed the functionality of Fap through Fap-related enzyme activity assay, cell invasion and migration experiments. Meanwhile, the cell line was also tested in in vivo mouse models, showing consistent results.

Does Fap expression have any effect on cell growth or behavior? Is there any relevant experimental data?

A: We conducted cell proliferation, apoptosis and migration experiments, and the results showed that Fap expression had no significant effect on the growth and behavior of CT26 cells. Experimental data, including cell proliferation curves, apoptosis analysis, and migration assay results, all support this conclusion.

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Customer Reviews

Consistent expression

Recently, I bought the stable cell line CT26 from Mouse Fap for my study on the dynamics of the tumor microenvironment. The cell line's ability to express Fibroblast Activation Protein (Fap) consistently was crucial to my research because it enabled me to learn more about the complex relationships that exist between tumor cells and the surrounding stroma. The derived CT26 cells were a dependable starting point for experiments because they adhered to culture surfaces well and kept their epithelial shape. All things considered, this product's performance and consistency have impressed me, and it has greatly advanced my research goals.

Canada

2024-04-26

dependable wording

For the cancer research in my lab, the Mouse Fap stable cell line CT26 from Creative Biogene has changed everything. Its reliable and accurate expression of Fibroblast Activation Protein (Fap) has allowed us to investigate the intricate interactions between tumor cells and the surrounding stroma.

Germany

2021-06-08

Interactions between tumor andstroma

For those doing research on the interactions between tumors and thestroma, this CT26 cell line is essential. Reliable outcomes are guaranteed by its stable Fap expression.

United Kingdom

2022-12-19

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Mouse Fap Stable Cell Line - CT26

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Documents

Background

Case Study

Applications

Q & A

Customer Reviews

How stable is Mouse Fap expression? Is there detailed stability experimental data?

Has this cell line been functionally validated? Has it been tested in in vivo or in vitro experiments?

Does Fap expression have any effect on cell growth or behavior? Is there any relevant experimental data?

Consistent expression

dependable wording

Interactions between tumor andstroma

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