Cat.No. : CSC-RO0690 Host Cell: CT26
| Cat. No. | CSC-RO0690 |
| Description | This cell line is engineered to stably overexpress mouse fibroblast activation protein (Fap) in CT26. |
| Gene | Fap |
| Host Cell | CT26 |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
The researchers used genetically encoded FAPdL5**, targeted to various subcellular locations, to bind a heavy atom-substituted dye (MG2I-ester), forming an on-demand TAP-producing singlet oxygen. Stable HEK-293 cell lines expressing FAP in distinct compartments were generated, selected by FACS for equal expression, and verified by fluorescence microscopy. Photocytotoxicity was assessed using MTS assays and cell death was characterized by flow cytometry post-illumination. FAP locations included cytoplasm, ER lumen, membrane, mitochondria, and nucleus.
Figure 1. HEK cells and CT26 cells with mitochondrial localization of FAP have significantly different sensitivities to singlet oxygen generation. CT26 cells show significant resistance to singlet oxygen generated by mitochondria. (Liang P, et al., 2020)
Using Creative Biogene's Mouse Fap Stable Cell Line - CT26 allows improved experiments to study the role of FAP in tumor cells with a CT26 cell line that more closely approximates cancer biology. This improvement improves the stability and consistency of experiments and helps to better understand the potential of FAP in cancer treatment.
A: Mouse Fap Stable Cell Line-CT26 has been tested for multiple passages and Fap expression remains stable. We conducted experiments with multiple consecutive passages and verified the expression level of Fap through qPCR and Western Blot. The results showed that there was no significant change in the expression of Fap.
A: This cell line has been functionally verified in vitro, proving that the Mouse Fap it expresses has biological activity. We confirmed the functionality of Fap through Fap-related enzyme activity assay, cell invasion and migration experiments. Meanwhile, the cell line was also tested in in vivo mouse models, showing consistent results.
A: We conducted cell proliferation, apoptosis and migration experiments, and the results showed that Fap expression had no significant effect on the growth and behavior of CT26 cells. Experimental data, including cell proliferation curves, apoptosis analysis, and migration assay results, all support this conclusion.
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Recently, I bought the stable cell line CT26 from Mouse Fap for my study on the dynamics of the tumor microenvironment. The cell line's ability to express Fibroblast Activation Protein (Fap) consistently was crucial to my research because it enabled me to learn more about the complex relationships that exist between tumor cells and the surrounding stroma. The derived CT26 cells were a dependable starting point for experiments because they adhered to culture surfaces well and kept their epithelial shape. All things considered, this product's performance and consistency have impressed me, and it has greatly advanced my research goals.
For the cancer research in my lab, the Mouse Fap stable cell line CT26 from Creative Biogene has changed everything. Its reliable and accurate expression of Fibroblast Activation Protein (Fap) has allowed us to investigate the intricate interactions between tumor cells and the surrounding stroma.
For those doing research on the interactions between tumors and thestroma, this CT26 cell line is essential. Reliable outcomes are guaranteed by its stable Fap expression.
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