cDNA Library Construction Service

cDNA libraries are essential tools and starting points for gene function studies, novel gene discovery, transcriptome analysis, and protein interaction research. Leveraging decades of expertise in molecular biology, Creative Biogene provides comprehensive, high-throughput, and high-standard one-stop services for cDNA library construction. Our platform standardizes and streamlines the complex library construction workflow, ensuring that clients receive high-quality, high-coverage cDNA libraries, enabling precise downstream analysis without the burden of labor-intensive experiments.

Why Choose Creative Biogene for cDNA Library Construction?

A high-quality cDNA library is crucial for success in functional genomics research. Traditional workflows are technically challenging, time-consuming, and highly sensitive to errors at any step, which can compromise the entire library. Creative Biogene's one-stop platform addresses these critical challenges with four core advantages:

Exceptional Library Quality

Primary libraries exceed 1×106 CFU with >95% recombination rate, allowing efficient directional cloning into standard or customized vectors, ensuring reliable capture of rare transcripts and robust downstream functional analysis.

Integrated and Optimized Workflow

From total RNA QC, mRNA purification, cDNA synthesis and modification, to vector ligation, transformation, and library quality control, we provide seamless end-to-end services. Clients need only supply samples or RNA to obtain a high-quality final library.

Flexible, Customizable Solutions

We offer eight major types of cDNA library construction to meet diverse research needs, from standard libraries to specialized libraries with normalization, subtraction, or other modifications.

Experience-Driven Professional Support

Our team of senior scientists has constructed thousands of successful libraries. From initial project design to troubleshooting and final data analysis, we provide comprehensive professional support.

Core Technology Platform

Creative Biogene's proprietary cDNA library construction and optimization platform integrates advanced enzymology, efficient nucleic acid handling, and intelligent bioinformatics analysis. This approach addresses traditional limitations in full-length capture, uniformity, and representativeness, delivering libraries suitable for downstream research.

Patented Normalization Algorithm

Balances high- and low-abundance transcripts, facilitating rare gene discovery.

Efficient Subtractive Hybridization System

Rapidly enriches differentially expressed genes, improving research efficiency.

Optimized Yeast Systems

Libraries designed for yeast single, double, or triple hybrid assays retain high functional activity in eukaryotic systems.

Comprehensive cDNA Library Types

Library Type	Key Features	Scientific Applications
Standard cDNA	Fast, economical, reliable	Snapshot of gene expression in specific tissues or cells
Full-Length cDNA	High full-length rate, complete 5' and 3' ends	Obtain complete ORFs for eukaryotic expression, protein production, and functional studies
Normalized cDNA	Balanced distribution of high- and low-abundance genes	Efficient screening of rare transcripts, reduced repetitive screening of abundant genes
Subtracted cDNA	Enrichment of differentially expressed genes	Identify genes with expression differences between samples
Suppression Subtractive Hybridization	Efficient enrichment of rare differentially expressed genes without physical separation	Enrich low-abundance DEGs, suitable for small sample sizes
Yeast One-Hybrid	Designed for protein-DNA interaction screening	Identify transcription factors binding specific DNA sequences
Yeast Two-Hybrid	Designed for protein-protein interaction screening	Identify prey proteins interacting with bait proteins at the genome-wide level
Yeast Three-Hybrid	Designed for interactions dependent on a third molecule	Study RNA- or protein-mediated complex protein interactions

Not sure which library suits your needs? Contact our scientists for a free consultation.

Case Study

Interactome of MGF360-21R and A151R in ASFV-infected HEK293 cells

Exploring Virus–Host Interactions Through Combined Proteomic Approaches Identifies BANF1 as a New Essential Factor for African Swine Fever Virus

Paper 2025

Molecular & Cellular Proteomics | DOI

Background
African swine fever virus (ASFV) causes deadly disease in pigs and threatens global pork production. This study combined affinity purification–mass spectrometry and yeast two-hybrid screening to investigate ASFV proteins MGF360-21R and A151R, identifying host protein BANF1 as a common and critical interactor. Silencing BANF1 significantly reduced ASFV replication, indicating a proviral role. Furthermore, both viral proteins suppressed type I interferon induction, while recombinant viruses lacking these genes induced stronger IFN responses in porcine macrophages. These findings illuminate ASFV host manipulation and highlight BANF1 as a potential antiviral target.
Key Findings
Using AP-MS and Y2H, 232 and 33 host interactors were identified for MGF360-21R and A151R, respectively, with 48 shared partners including BANF1. GST pulldown and colocalization confirmed interactions, and MST determined a Kd of 105 nM for A151R-BANF1. Expression of either viral protein caused relocalization of nuclear BANF1 to cytoplasm. BANF1 knockdown in WSL cells reduced ASFV titers by approximately 2 logs and altered expression of proteins in innate immune and chromatin-remodeling pathways. Luciferase assays showed that A151R and MGF360-21R inhibit IFN-β induction by cGAS/STING, poly(dA:dT), or RIG-I without affecting ISRE. Deletion mutants of either gene in ASFV Georgia/07 triggered higher IFN-α and CXCL10 secretion in infected macrophages than wild-type, and ΔA151R exhibited a replication defect. These results uncover new immune evasion functions and establish BANF1 as a necessary host factor.
Contribution
Creative Biogene provided the porcine cDNA library used in yeast two-hybrid screens.

In a study of host dependency factors for multiple SARS-CoV-2 variants, researchers found conserved transcriptional responses in infected Calu-3 cells and, using a genome-wide CRISPR loss-of-function screen, identified host genes required for infection, many shared across variants; validation with small molecules and approved drugs highlighted reactive oxygen species production as an early step in viral replication. Creative Biogene's cDNA library construction platform can support similar studies by providing full-length, normalized, and differentially enriched cDNA libraries suitable for capturing transcriptomic changes and enabling high-confidence discovery of genes involved in variant-independent cellular responses.

Figure 1. High-stringency CRISPR loss-of-function screening in Calu-3 cells using the GeCKO v2 library (Creative Biogene) identified host genes involved in SARS-CoV-2 variant infection. (Frasson I, et al., 2024)

Start Your Gene Discovery Journey

Streamline your research by choosing Creative Biogene's reliable, professional, and experienced cDNA library construction services. Contact us for a free project evaluation and quotes. Our expert team is ready to help transform your innovative ideas into scientific discoveries.

FAQ

What materials are needed to start a cDNA library project?

Sample: Fresh-frozen tissue (>100 mg), cells (>10^7), or high-quality total RNA (>200 ng/µL, RIN >8.0, intact). Total RNA is preferred.
Project Info: Experimental goal, library type, downstream applications (e.g., yeast hybrid, phage display), and any specific vector.
Our scientific advisors provide guidance on sample preparation and design if uncertain.

Typical construction timeline for different library types:

The typical construction timeline depends on the library type: standard cDNA libraries generally take about four to five weeks, full-length, normalized, subtracted, or SSH libraries require approximately six to eight weeks, and yeast hybrid libraries usually take five to seven weeks. Timelines may vary based on sample quality and specific library requirements, and detailed schedules are provided at the beginning of each project.

Are downstream screening or sequencing services provided?

Constructed cDNA libraries are ready for downstream applications, including high-throughput sequencing using Illumina or PacBio platforms, functional screening such as yeast one-, two-, and three-hybrid assays, or phage display selection, and targeted gene cloning directly from the library.