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In the field of genomics and functional gene research, the fosmid library has become a reliable medium-insert cloning tool that combines high stability and precision. It plays an important role in genome gap filling, physical map construction, positional cloning, metagenomic studies, and functional screening.
Figure 1. Schematic workflow of vector library construction.
As a global leader in molecular biology solutions, Creative Biogene provides high-quality, customizable fosmid library construction services, covering every step from sample preparation to library validation, ensuring accuracy, stability, and reproducibility for both academic and industrial clients.
Fosmid vectors are derived from the Escherichia coli F plasmid, which shares a similar low-copy replication system with BACs (Bacterial Artificial Chromosomes). This low-copy feature minimizes the risk of insert recombination or loss, ensuring exceptional clone stability. Compared with cosmids (~40 kb inserts), fosmids exhibit superior genetic stability and a more uniform fragment distribution.
Typical fosmid inserts range from 35–45 kb, providing high coverage for complex genomes. Random cloning, low background noise, and efficient construction make fosmid libraries particularly suitable for positional gene mapping and physical map assembly.
Low-copy, high stability
Maintains single-copy state to prevent recombination events.
Inducible high-copy feature
The oriV origin enables temporary copy amplification (~50 copies) for convenient DNA extraction.
Uniform fragment distribution
Physical random shearing ensures unbiased insert representation.
Strong scalability
Compatible with high-throughput clone screening, end sequencing, and metagenomic assembly.
Creative Biogene's optimized fosmid library platform uses high-molecular-weight DNA, random physical shearing, and an efficient cloning system to ensure comprehensive genome coverage and reliable quality, with flexible customization for specific project needs.
Creative Biogene provides various fosmid library construction options tailored to specific research goals:
All procedures follow strict laboratory standards ensuring reproducibility and traceability.
01 Typical Quality Metrics
02 Deliverables
Due to its stability, insert size range, and short construction cycle, the fosmid library is widely used in:
Fosmid libraries also serve as valuable tools in gene discovery, molecular breeding, and industrial strain improvement.
Creative Biogene is committed to delivering efficient and reliable genomic cloning solutions to research and industrial clients worldwide. Our fosmid library construction service can be tailored to support metagenomic assembly, positional cloning, or structural variation analysis—helping you obtain high-quality, stable, and comprehensive fosmid libraries for your project. Contact our technical team today to discuss your research goals and receive a customized fosmid library proposal and quotation.
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