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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | The JeKo-1 mantle cell lymphoma (MCL) cell line was isolated from peripheral blood mononuclear cells of a 78-year-old woman with large-cell type mantle cell lymphoma that had undergone leukemic transformation. JeKo-1 cells were Epstein-Barr virus negative and showed a B-cell phenotype with IgM+, IgD+, CD3−, CD5+, CD10−, CD19+, CD20+ and CD23−. They overexpressed cyclin Dl, Bcl-2, c-Myc and Rb proteins. JeKo-1 cells showed complex chromosomal aberrations, but cytogenetically lacked an apparent t(11;14)(q13;q32) chromosomal translocation. However, bcl-1/JH gene rearrangement was confirmed at the molecular level, and overexpression of PRAD1/cyclin D1 protein was revealed in JeKo-1 cells and the original BM lymphoma cells. The JeKo-1 cells are highly tumorigenic in SCID mice and have applications for immunology research. |
| Tissue | Peripheral blood |
| Disease | Lymphoma |
| Morphology | Lymphoblast |
| Gender | Female |
| Age | 78 years |
| Product Format | Frozen |
| Growth Mode | Suspension |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Drug development 2. Mechanism study of mantle cell lymphoma 3. Genetics and genomics studies 4. Immunotherapy research 5. Biomarker discovery 6. Basic cancer research |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | p53 mutations and protein overexpression have been associated with poor prognosis or blastic variants of MCL. A substantial number of JeKo-1 cells were large or anaplastic in morphology, and the original tumour showed an aggressive clinical course, but JeKo-1 cells did not overexpress p53 protein. JeKo-1 cells expressed Rb, c-Myc and Bcl-2 instead. Zuckerberg et al demonstrated that most cases of MCL express both cyclin Dl and Rb. Bcl-2 has been shown to be consistently expressed in MCL. These results were consistent with the present data. |
| Characteristics | |
| Antigen Expression | CD3-, CD5+, CD10+, CD19+ |
| Tumorigenic | Yes, JeKo-1 cells are highly tumorigenic in SCID mice. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | Homogenize the cell suspension in the flask by gently pipetting up and down, then take a representative sample to determine the cell density per milliliter. Dilute the suspension with fresh medium to a concentration of 1 x 10^5 cells/mL and aliquot the adjusted suspension into new flasks for continued culture. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | The ratio of 1:2 to 1:4 is recommended. |
| Culture Medium | 80% RPMI 1640 + 20% h.i. FBS |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Frozen with 70% medium, 20% FBS, 10% DMSO |
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