Cat.No. : CSC-RO0705 Host Cell: KB
| Cat. No. | CSC-RO0705 |
| Description | This cell line is engineered to stably overexpress human FAP in KB which is a human epithelial carcinoma cell line and is commonly used in the field of oncology. |
| Gene | FAP |
| Gene Species | Homo sapiens (Human) |
| Host Cell | KB |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Among malignant tumors, hepatocellular carcinoma (HCC) has a high incidence rate. Hepatic stellate cells (HSCs) were the focus of the researchers' investigation into the functions of IL-17a and FAP in liver fibrosis and carcinogenesis. To evaluate the effects of IL-17a administration, IL-17a overexpression, and FAP upregulation in HSCs, they conducted both in vitro and in vivo investigations. The binding of STAT3 to the FAP promoter was verified by the CUT&RUN approach. According to in vitro findings, IL-17a stimulated HSCs, aiding in the onset and spread of hepatocellular carcinoma (HCC). Overexpression of FAP and IL-17a also promoted HSC activation, migration, and proliferation of HCC cells while preventing apoptosis. According to in vivo research, HSC overexpression of FAP and IL-17a promoted the growth of liver tumors. The study found that by activating STAT3, IL-17a increases FAP expression in HSCs, which in turn promotes HCC.
Figure 1. The researchers used lentiviral transfection to construct a FAP cell line that promoted LX2 cell proliferation and migration and inhibited apoptosis. Methods include CCK8, EdU, Transwell, scratch, TUNEL, and other experiments. (Sun D, et al., 2024)
A: The FAP expression of Human FAP Stable Cell Line-KB has been verified in detail, including qPCR, Western Blot and flow cytometry. qPCR showed that there was no significant difference in the expression level of FAP mRNA in different passages, and Western Blot and flow cytometry results also showed that the expression of FAP protein was stable. Specific experimental data can be provided upon request.
A: Human FAP Stable Cell Line-KB is suitable for FAP-related cancer research, drug screening and immunology research. Functional verification includes FAP enzyme activity measurement, cell invasion and migration experiments. The results show that the FAP expressed by this cell line has biological activity and is suitable for related research.
A: We conducted cell proliferation, invasion and migration experiments, and the results showed that FAP expression has a certain impact on the growth rate and invasion ability of KB cells. The specific manifestation is that the invasion ability of FAP-expressing cells is enhanced and the proliferation rate is slightly increased. These data can provide reference in experimental design to ensure the accuracy of experimental results.
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It is very easy to culture with KB cells, without the need for complex techniques and equipment. These cells show excellent adhesion and growth properties and can be easily cultured using traditional cell culture methods.
The delivery speed is fast, and the growth characteristics of KB cells make them an ideal model for cancer research. Rapid cell proliferation and stability of cell morphology were observed.
KB cells show good stability, which allows them to be reliably cultured in a laboratory environment and provides a solid foundation for research. During the culture process, we observed consistent cell growth and stable gene expression.
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