Mouse Fap Stable Cell Line - B16F10

Name: Mouse Fap Stable Cell Line - B16F10
SKU: CSC-RO0701
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0701

Host Cell : B16F10 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

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Cat. No.	CSC-RO0701
Description	This cell line is engineered to stably overexpress mouse Fap in B16F10 which is a murine melanoma cell line and is commonly used in the field of oncology.
Target Gene	Fap
Gene Species	Mus musculus (Mouse)
Host Cell	B16F10
Host Cell Species	Mus musculus (Mouse)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Fap fibroblast activation protein [ Mus musculus ]
Gene Symbol	Fap
Gene Description	fibroblast activation protein
GeneID	14089
Uni ProtID	A2AS87
mRNA Refseq	NM_007986.2
Protein Refseq	NP_032012.1
Chromosome Location	2 C1.3; 2 36.0 cM
Function	endopeptidase activity; hydrolase activity; peptidase activity; peptidase activity; protease binding; serine-type endopeptidase activity; serine-type peptidase activity;

CSC-RO0701-Mouse Fap Stable Cell Line - B16F10-Datasheet

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Background

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The Mouse Fap Stable Cell Line, specifically the B16F10 cell line, is derived from a mouse melanoma and is known for its high expression of FAP (Fibro/adhesive protein). FAP is a cell surface serine protease that is involved in tissue remodeling and has been implicated in cancer progression, particularly in melanoma. It is often upregulated in tumor-associated fibroblasts and has been shown to promote tumor growth, invasion, and metastasis. The B16F10 cell line, which is widely used in cancer research, provides a model for studying the role of FAP in melanoma and other cancers. It is also used to test the efficacy of anti-cancer drugs and therapies targeting FAP, offering insights into potential clinical applications for FAP inhibitors.

The Mouse Fap Stable Cell Line - B16F10 is a model for studying the role of FAP in cancer progression and metastasis. Its applications include:

(1) Tumor Microenvironment Interactions: FAP is expressed in tumor-associated fibroblasts and contributes to the tumor microenvironment. This cell line can be used to study how FAP influences tumor growth, angiogenesis, and immune evasion, providing insights into the complex interactions between cancer cells and their surroundings.

(2) Metastasis and Invasion: FAP has been implicated in promoting cancer cell invasion and metastasis. Researchers can use this cell line to investigate the role of FAP in these processes, potentially leading to the development of anti-metastatic strategies.

(3) Therapeutic Targeting: Given the upregulation of FAP in certain cancers, this cell line can be utilized to test the efficacy of FAP inhibitors or other targeted therapies. By evaluating the impact of these treatments on FAP-expressing cancer cells, researchers can determine the potential of FAP as a therapeutic target.

Customer Q&As

How can the Mouse Fap Stable Cell Line - B16F10 be used to study the role of autophagy in tumor cells?

A: The B16F10 cell line can be used to study the role of autophagy in tumor cell survival and stress adaptation. By monitoring the expression of autophagy-related genes and proteins, researchers can explore the function of autophagy in tumor biology.

What is the application of the Mouse Fap Stable Cell Line - B16F10 in studying the relationship between tumor cells and angiogenesis?

A: By observing the behavior of B16F10 cells in in vitro angiogenesis models, such as tube formation assays, researchers can understand how tumor cells promote angiogenesis, which is essential for tumor growth and metastasis.

How can the Mouse Fap Stable Cell Line - B16F10 be utilized to study the epigenetic regulation of tumor cells?

A: The B16F10 cell line can be used to study how epigenetic events, such as DNA methylation and histone modifications, affect gene expression and function in tumor cells. This is crucial for understanding the epigenetic regulatory network in tumor development.

How can the Mouse Fap Stable Cell Line - B16F10 be applied in studying the apoptotic mechanisms of tumor cells?

A: By inducing apoptotic stimuli and monitoring apoptotic markers, such as Caspase activity, the B16F10 cell line can be used to study the molecular mechanisms of tumor cell apoptosis, which is significant for developing new anti-tumor drugs.

What role does the Mouse Fap Stable Cell Line - B16F10 play in studying the mechanisms of tumor invasion and metastasis?

A: The B16F10 cell line is commonly used to study the invasiveness and metastatic capabilities of tumors. By observing the behavior of these cells in in vitro invasion models, such as Matrigel invasion assays, researchers can understand how tumor cells penetrate the basement membrane and extracellular matrix.

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Customer Reviews

Immune Response Study

This cell line helps in researching the interactions between tumors and the host immune system.

United Kingdom

2020-05-13

Biomarker Development

Through the B16F10 cell line, biomarkers for tumor therapy can be developed and validated.

French

2024-01-29

Gene Function Analysis

The B16F10 cell line is suitable for studying the role of specific genes in tumorigenesis.

United Kingdom

2021-01-26

Tumor Research Model

The Mouse Fap Stable Cell Line - B16F10 is a vital cell line for studying melanoma, aiding in understanding tumor growth and metastasis mechanisms.

Germany

2022-07-29

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Mouse Fap Stable Cell Line - B16F10

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Documents

Background

Applications

Q & A

Customer Reviews

How can the Mouse Fap Stable Cell Line - B16F10 be used to study the role of autophagy in tumor cells?

What is the application of the Mouse Fap Stable Cell Line - B16F10 in studying the relationship between tumor cells and angiogenesis?

How can the Mouse Fap Stable Cell Line - B16F10 be utilized to study the epigenetic regulation of tumor cells?

How can the Mouse Fap Stable Cell Line - B16F10 be applied in studying the apoptotic mechanisms of tumor cells?

What role does the Mouse Fap Stable Cell Line - B16F10 play in studying the mechanisms of tumor invasion and metastasis?

Immune Response Study

Biomarker Development

Gene Function Analysis

Tumor Research Model

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