Cat.No. : CSC-RO0701 Host Cell: B16F10
| Cat. No. | CSC-RO0701 |
| Description | This cell line is engineered to stably overexpress mouse Fap in B16F10 which is a murine melanoma cell line and is commonly used in the field of oncology. |
| Gene | Fap |
| Gene Species | Mus musculus (Mouse) |
| Host Cell | B16F10 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The B16F10 cell line can be used to study the role of autophagy in tumor cell survival and stress adaptation. By monitoring the expression of autophagy-related genes and proteins, researchers can explore the function of autophagy in tumor biology.
A: By observing the behavior of B16F10 cells in in vitro angiogenesis models, such as tube formation assays, researchers can understand how tumor cells promote angiogenesis, which is essential for tumor growth and metastasis.
A: The B16F10 cell line can be used to study how epigenetic events, such as DNA methylation and histone modifications, affect gene expression and function in tumor cells. This is crucial for understanding the epigenetic regulatory network in tumor development.
A: By inducing apoptotic stimuli and monitoring apoptotic markers, such as Caspase activity, the B16F10 cell line can be used to study the molecular mechanisms of tumor cell apoptosis, which is significant for developing new anti-tumor drugs.
A: The B16F10 cell line is commonly used to study the invasiveness and metastatic capabilities of tumors. By observing the behavior of these cells in in vitro invasion models, such as Matrigel invasion assays, researchers can understand how tumor cells penetrate the basement membrane and extracellular matrix.
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This cell line helps in researching the interactions between tumors and the host immune system.
Through the B16F10 cell line, biomarkers for tumor therapy can be developed and validated.
The B16F10 cell line is suitable for studying the role of specific genes in tumorigenesis.
The Mouse Fap Stable Cell Line - B16F10 is a vital cell line for studying melanoma, aiding in understanding tumor growth and metastasis mechanisms.
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