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Mutant Library Construction Service

As one of the most competitive biotechnology companies in the world, Creative Biogene has extensive expertise and experience in providing you with custom mutant library construction services. With the help of sophisticated equipments, advanced technologies and highly experienced staffs, Creative Biogene can provide you with a range of mutant libraries construction service, including site-directed mutagenesis library, scanning point mutation library and randomized and degenerated library. Creative Biogene’s goal is to provide you with the most affordable and high-quality mutant library construction services to ensure your satisfaction in a timely and professional manner.

A mutant library is a collection of protein variants which is becoming more and more popular in identification of vital residues controlling physiological activities and the investigation of protein optimization. Creative Biogene which possesses high-throughput gene synthesis and mutagenesis platform and highly experienced staffs is able to provide the most affordable mutant library construction services with fastest turnaround time to satisfy your downstream needs.

Creative Biogene is able to provide the following mutant library construction services for your scientific research:

Site-directed Mutagenesis Libraries

By combining de novo gene synthesis and site-directed mutagenesis technique, Creative Biogene can offer the most advanced site-directed mutagenesis library construction services. In these libraries, any given residue can be substituted with any of other 19 common amino acids.

Scanning Point Mutation Libraries

Scanning point mutation is a systematic means of improving protein performance. It outperforms standard alanine/cysteine scanning that it is able to substitute each amino acid with all 20 amino acids simultaneously. This technique enables to provide a detailed profile of each amino acid at the position.

Randomized and Degenerated Libraries

With the help of advanced degenerate oligonucleotide techniques, Creative Biogene can construct any form of randomization or degenerate of full-length gene in an interested DNA fragment. The mutation frequency can be set to any value between 1 and 12 mutations per kb.

References:

  1. Laible M, Boonrod K. Homemade site directed mutagenesis of whole plasmids. JoVE, 2009 (27): e1135-e1135.
  2. Morrison K L, Weiss G A. Combinatorial alanine-scanning. Current opinion in chemical biology, 2001, 5(3): 302-307.
  3. Cadwell R C, Joyce G F. Randomization of genes by PCR mutagenesis. Genome research, 1992, 2(1): 28-33.

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