Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RO0707
Host Cell : C4-2 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RO0707 |
| Description | This cell line is engineered to stably overexpress human FAP in C4-2 which is a human prostate cancer cell line derived from LNCaP cell subcutaneous xenograft tumor of castrated mouse. |
| Target Gene | FAP |
| Gene Species | Homo sapiens (Human) |
| Host Cell | C4-2 |
| Host Cell Species | Homo sapiens (Human) |
| Applications |
1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Size | >1x106 frozen cells/vial |
| Stability | Validated for at least 10 passages |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Gene Name | FAP fibroblast activation protein, alpha [ Homo sapiens ] |
| Gene Symbol | FAP |
| Synonyms | FAP; fibroblast activation protein, alpha; seprase; DPPIV; integral membrane serine protease; 170 kDa melanoma membrane-bound gelatinase; FAPA; DKFZp686G13158; |
| GeneID | 2191 |
| Uni ProtID | Q12884 |
| mRNA Refseq | BC026250 |
| Chromosome Location | 2q23 |
| Function | dipeptidyl-peptidase activity; endopeptidase activity; metalloendopeptidase activity; peptidase activity; protease binding; protein binding; protein dimerization activity; protein homodimerization activity; serine-type endopeptidase activity; serine-type peptidase activity; contributes_to serine-type peptidase activity; serine-type peptidase activity; |
| MIM | 600403 |
The Fap gene, or Fibroblast Activation Protein gene, codes for a secreted protein that participates in multiple biological processes such as cell proliferation, migration, and invasion. Abnormal expression of the Fap gene has been associated with the development of various diseases, particularly cancer. Inhibition of the Fap gene or its protein product has been shown to suppress tumor growth and metastasis in preclinical models, making it a potential target for cancer therapy.
The C4-2 Cell Line is a prostate cancer cell line that has been widely used in prostate cancer research. It is derived from the LNCaP cell line and is considered to be more aggressive and invasive. C4-2 cells express high levels of androgen receptor and PSA, which are key markers for prostate cancer. This cell line is commonly used in preclinical studies to investigate the efficacy of novel anticancer agents and to understand the molecular mechanisms of prostate cancer development and progression. Due to its relevance to human prostate cancer, the C4-2 cell line has contributed significantly to the advancement of our understanding of this disease and the development of potential therapeutic strategies.
The Human FAP Stable C4-2 Cell Line is a valuable tool for researchers investigating the role of Fibromodulin (FAP) in tumorigenesis, angiogenesis, and metastasis. This stable cell line, which overexpresses FAP, offers a controlled experimental system to study the functional significance of FAP in various aspects of cancer progression.
Clinically, the Human FAP Stable C4-2 Cell Line can aid in the development of novel diagnostic and therapeutic strategies for cancer patients. By studying the behavior of these cells, researchers can identify potential biomarkers for tumor detection, prognosis, and treatment response. Additionally, understanding the role of FAP in angiogenesis and metastasis may lead to the development of targeted therapies that inhibit these processes, thereby improving patient outcomes.
In research, the Human FAP Stable C4-2 Cell Line can be used to investigate the underlying mechanisms of FAP’s involvement in tumor growth and metastasis. By manipulating FAP expression and activity in these cells, researchers can gain insights into the signaling pathways and molecular interactions that regulate these processes. This knowledge can contribute to the development of novel targeted interventions to prevent or treat cancer.
Furthermore, the Human FAP Stable C4-2 Cell Line can be employed to study the role of FAP in drug resistance and to identify potential therapeutic strategies to overcome this resistance. By testing various compounds and drugs on these cells, researchers can identify novel drug targets and develop combination therapies that enhance treatment efficacy.
In summary, the Human FAP Stable C4-2 Cell Line has diverse applications in clinical and research settings, providing valuable insights into the role of FAP in cancer progression and offering potential avenues for the development of novel diagnostic and therapeutic approaches.
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The Human FAP Stable Cell Line - C4-2 is a versatile tool for various applications, including studying the functions of the FAP gene in cancer biology, drug development, and disease modeling. Its wide range of uses makes it an invaluable resource for researchers across different scientific disciplines.
The Human FAP Stable Cell Line - C4-2 is produced and distributed in compliance with all relevant laws and regulations regarding intellectual property. This ensures that researchers can use the cell line without legal concerns, promoting ethical and responsible scientific research practices.
The production and use of the Human FAP Stable Cell Line - C4-2 prioritize environmental sustainability, employing eco-friendly practices in cell culture and waste management. This commitment to reducing ecological footprint aligns with responsible research and contributes to the overall sustainability of scientific exploration.
The Human FAP Stable Cell Line - C4-2 is widely accepted within the scientific community, reflecting its significant contribution to advancing medical knowledge and understanding of disease mechanisms. Its use is seen as a positive step towards improving human health and well-being, making it a socially beneficial research tool.
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