Cat.No. : CSC-RO0704 Host Cell: HEK293
| Cat. No. | CSC-RO0704 |
| Description | This cell line is engineered to stably co-express human FAP and FOLH1 in HEK293. |
| Gene | FAP/FOLH1 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Cribriform prostate cancer, found in invasive cribriform carcinoma (ICC) and intraductal carcinoma (IDC), is a highly aggressive subtype associated with lethal progression. Researchers investigate the aggressiveness of cribriform prostate cancer (ICC/IDC) using single-cell RNA sequencing and histology on paired tumor and benign samples. They find ICC/IDC cells express metastasis-associated genes and potential therapeutic targets. The tumor microenvironment (TME) is characterized by increased angiogenesis, immunosuppressive fibroblasts (CTHRC1+ASPN+FAP+ENG+), and dysfunctional T cells. These intrinsic pathways and immunosuppressive TME components contribute to ICC/IDC aggressiveness, suggesting potential therapeutic avenues to restore immune signaling for better outcomes.
Figure 1. Researchers quantified FAP/FOLH1 expression in a prostate cancer cohort, observing its levels in different histological patterns. (Wong HY, et al., 2022)
If utilizing Creative Biogene's Human FAP/FOLH1 stable cell line, the pivotal role in the proliferation, migration, and invasion of ICC/IDC cells can be assessed. Opting for our product for similar experiments enables in-depth investigation into the role of FAP/FOLH1 in tumor development, thereby providing reliable experimental support for novel strategies in cancer therapy.
A: This stable cell line combines the expression of FAP and FOLH1, enabling investigation into their individual or synergistic roles in tumor progression, drug targeting, and immune modulation.
A: Expression was verified through immunoblotting, flow cytometry, or qPCR assays, with selection pressure applied to ensure stable maintenance of transgene expression.
A: Functional studies may include assays evaluating enzymatic activity, substrate specificity, cellular proliferation, migration, or interactions with the tumor microenvironment.
A: The cell line serves as a valuable tool for assessing the efficacy and specificity of FAP/FOLH1-targeted therapeutics, facilitating the identification of potential candidates for further preclinical or clinical evaluation.
A: Comparative analysis with clinical specimens or animal models helps validate the relevance of this stable cell line in mimicking physiological or pathological conditions, guiding translational research efforts.
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This Human FAP/FOLH1 Stable Cell Line is a game-changer! Its dual stable expression lets me tackle complex cancer research with confidence.
Using this cell line has been a breeze! The stable expression of FAP and FOLH1 in HEK293 cells has made my experiments smoother and more reliable.
Can't believe how much easier my work has become with this cell line! Its dual stable expression of FAP and FOLH1 has opened up new possibilities in cancer research.
So impressed with this Human FAP/FOLH1 Stable Cell Line! Its consistent expression of FAP and FOLH1 has given me peace of mind and boosted the quality of my data.
Huge thanks to this cell line for simplifying my research! With stable expression of both FAP and FOLH1, I can delve deeper into tumor microenvironment interactions without any hassle.
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