Cat.No. : CSC-RR0287 Host Cell: SK-BR-3
| Cat. No. | CSC-RR0287 |
| Description | SK-BR-3 - RFP cell line has been developed through stable transfection with RFP. RFP is expressed as free cytoplasmatic proteins in SK-BR-3 - RFP cells. This cell line is a ready-to-use in vitro model for cell-based assay applications. |
| Gene | RFP |
| Host Cell | SK-BR-3 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Source | SK-BR-3 |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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The cells exhibit consistent and robust red fluorescence, which allowed us to easily track gene expression and cellular behaviors in our breast cancer research.
The technical support we received for the RFP Reporter Cell Line - SK-BR-3 has been outstanding. The team was responsive and knowledgeable, addressing our queries and providing detailed guidance.
As researchers, reproducibility is of utmost importance and the RFP reporter cell line - SK-BR-3 excels in this regard. We have performed multiple experiments using these cells and the results have been very consistent each time.
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