Cat.No. : CSC-RR0514 Host Cell: PC-9
| Cat. No. | CSC-RR0514 |
| Description | This cell line is engineered to stably overexpress GFP and luciferase reporter gene. PC-9 cell line is a human lung cancer cell line that has been widely used in laboratory research. This cell line is a useful tool for fluorescence and bioluminescence assays. |
| Gene | GFP/Luciferase |
| Host Cell | PC-9 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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Its dual reporter system allows us to monitor gene expression and cellular responses with exceptional precision and versatility in both in vitro and in vivo experiments.
We've applied GFP/Luc Reporter Cell Line - PC-9 in various studies, from drug screening to pathway analysis, and it consistently delivers reliable, reproducible results.
The robust expression of GFP provides excellent visual confirmation under fluorescence microscopy, while the luciferase activity offers quantifiable data with great linearity. This combination ensures that our assays are both qualitative and quantitative, enhancing the depth of our experimental insights.
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