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GFP Reporter Cell Line - PC-12

GFP Reporter Cell Line - PC-12

Cat.No. :  CSC-RR0284 Host Cell:  PC-12

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Cell Line Information

Cell Culture Information

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Cat. No. CSC-RR0284
Description PC-12 - GFP cell line has been developed through stable transfection with GFP. GFP is expressed as free cytoplasmatic proteins in PC-12 - GFP cells. This cell line is a ready-to-use in vitro model for cell-based assay applications.
Gene GFP
Host Cell PC-12
Host Cell Species Rattus norvegicus (Rat)
Stability Validated for at least 10 passages
Reporter Type Fluorescent protein
Application

1. Gene expression studies

2. Protein localization

3. Drug screening and toxicology

4. Live cell imaging

Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Storage Liquid nitrogen
Source PC-12
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations

The following safety precautions should be observed.

1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.

2. No eating, drinking or smoking while handling the stable line.

3. Wash hands after handling the stable line and before leaving the lab.

4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.

5. All waste should be considered hazardous.

6. Dispose of all liquid waste after each experiment and treat with bleach.

Ship Dry ice
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