Cat.No. : CSC-RR0486 Host Cell: MKN45
| Cat. No. | CSC-RR0486 |
| Description | Luciferase Reporter Cell Line - MKN45 is engineered to stably express luciferase encoding gene. This cell line is an ideal tool for bioluminescent tracing of MKN45 cells. MKN45 is a type of human gastric cell line that can be used in gastric cancer research. |
| Gene | Luc |
| Host Cell | MKN45 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Although its rates have recently decreased, gastric cancer (GC) still has a very high death rate globally. It is the fifth most prevalent disease in women and the fourth in males. Using the Luciferase Reporter Cell Line - MKN45, the researchers looked into how miR-135b affected the cisplatin resistance of gastric cancer (GC) cells. They used a dual luciferase reporter gene experiment to verify that miR-135b directly targets MST1, so linking the MAPK signaling pathway to the advancement of GC. The preparation, transfection, and testing of high CDDP-resistant GC cell lines in CDDP-treated nude mouse models demonstrated the crucial role MST1 plays in GC formation and drug sensitivity. The modulation of MST1 by miR-135b and its effect on GC pathogenesis are highlighted in this study.
Figure 1. The MKN45 Luciferase Reporter Cell Line was utilized by the researchers to exhibit how MST1 functions as a target gene for miR-135b. In MKN45 cells, they evaluated MST1-wild type and mutant constructs using dual luciferase reporter assays. (Zhou J, et al., 2019)
A: The MKN45 Luciferase reporter cell line has high-level and stable luciferase activity and is suitable for long-term experiments. This cell line has been optimized to maintain stable luciferase expression levels under different experimental conditions, making it suitable for use in a variety of biological research and drug screening. Its high sensitivity and low background noise make experimental results more reliable and accurate. In addition, the cell line can maintain stable luciferase activity during long-term culture and multiple passages, ensuring data consistency and experimental reproducibility.
A: To ensure optimal detection when performing luminescence experiments using the MKN45 Luciferase reporter cell line, there are several key points that should be noted. First, select an appropriate luminescent substrate (such as D-luciferin) and ensure its purity and quality to maximize the luminescent efficiency of luciferase. Secondly, accurately control the time and amount of substrate addition to avoid signal deviation caused by excess or deficiency. Third, use luminescence detection equipment with high sensitivity and low background noise, such as a microplate luminescence detector, and set appropriate measurement parameters (such as integration time, measurement mode, etc.). In addition, ensure healthy cell status and avoid signal fluctuations caused by poor cell status.
A: Answer: When processing and analyzing large amounts of luminescence data generated by the MKN45 Luciferase reporter cell line, the data should first be preprocessed, including removing outliers and background noise. Next, use professional data analysis software or statistical software to normalize the data to eliminate systematic errors between experiments. Then, the data are quantitatively analyzed through the standard curve or internal standard method to ensure the accuracy of the results. In order to verify the reliability of the results, it is recommended to repeat the experiment multiple times to obtain the average value and standard deviation. In addition, data can be presented in the form of charts or images to visually reflect experimental results and trends.
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The merchant's customer service team is very professional and patient. They can quickly answer all my questions and provide detailed product information and technical support. I received a lot of practical advice and help when selecting and using the Luciferase Reporter cell line MKN45. Will come again next time to buy other products.
The cells grew stably during the culture process and showed excellent adaptability under standard cell culture conditions. The luciferase reporter system allows me to sensitively quantify gene expression levels, which is very helpful for me in studying the molecular mechanisms of gastric cancer. Overall, this cell line is very reliable and meets my research needs.
The cells' gene expression is very responsive, allowing me to easily track changes in gene regulation under different treatments or stimuli. In addition, the tumor-forming ability and specific genetic abnormalities of the MKN45 cell line provide me with a valuable model for in-depth study of the pathogenesis of gastric cancer. This purchasing experience was very pleasant.
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