Cat.No. : CSC-RR0118 Host Cell: LNCAP
| Cat. No. | CSC-RR0118 |
| Description | The LNCap cell line is a cell line derived from a needle human prostate adenocarcinoma cells of a 50-year old caucasian male in 1977, where cells were taken from a needle aspiration biopsy of a metastatic lesion in the left supraclavicular lymph node. The GFP Stable Cell Line-LNCAP constitutively expresses GFP. |
| Host Cell | LNCAP |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Recommended Medium | Inquiry for instruction of culturing |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
The researchers discuss the different features of prostate cancer (PCa) cells dependent on PSA expression. PSA−/lo cells are resistant to stress, including androgen deprivation, have a high clonogenic capacity, and are tumorigenic over time. They express stem cell genes, divide asymmetrically to produce PSA+ cells, and initiate strong tumor growth in castrated animals. Importantly, they contain castration-resistant PCa cells with the ALDH+CD44+α2β1+ phenotype. PSA+ cells, on the other hand, have limited tumorigenicity, divide symmetrically, and are castration sensitive. This shows PSA−/lo cells are important donors of castration-resistant PCa cells.
Figure 1. The researchers describe distinct properties of PCa cells expressing high (PSA+) and low (PSA−/lo) levels of PSA. PSA−/lo cells are quiescent, resistant to stress like androgen deprivation, highly clonogenic, and possess long-term tumorigenic capacity. They express stem cell genes, undergo asymmetric division generating PSA+ cells, initiate robust tumor growth, resist androgen ablation, and harbor highly tumorigenic castration-resistant PCa cells enriched via ALDH+CD44+α2β1+ phenotype. (Qin J, et al., 2021)
A: GFP fluorescence allows for the real-time visualization and tracking of LNCaP cells when co-cultured with other cell types or within a three-dimensional matrix, enabling the study of interactions with stromal cells, extracellular matrix components, and the impact of the microenvironment on prostate cancer cell behavior.
A: Drug delivery systems can be labeled with a fluorescent tracer or conjugated to a compound that quenches GFP fluorescence upon interaction. The targeting accuracy can then be evaluated by fluorescence microscopy or flow cytometry, observing the colocalization or quenching of GFP in LNCaP cells.
A: Yes, androgen deprivation effects on LNCaP cells can be monitored by live-cell imaging, using GFP fluorescence to observe changes in cell morphology, proliferation, and apoptosis over time, providing insights into cellular dynamics under hormone-deprived conditions.
A: The stable GFP expression enables easy identification and sorting of LNCaP cells from a mixed population using flow cytometry. Subsequently, cell surface proteomics can be conducted to identify prostate cancer-specific markers, which can be validated by comparing GFP-positive cells with GFP-negative cells.
A: The cell line can be used in various assays, such as wound healing, transwell migration, and invasion assays, to study the mechanisms involved in prostate cancer metastasis. Additionally, intravital imaging in animal models can provide a visual assessment of metastatic behavior, tracking the migration of GFP-labeled LNCaP cells to distant sites.
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This cell line is hormone-sensitive, reflecting key aspects of hormone-responsive prostate cancers, which is critical for testing hormonal therapies.
The LNCAP cells adhere well to culture substrates, simplifying handling and enabling various in vitro assays without the need for suspension adaptation.
GFP Stable Cell Line-LNCAP expresses prostate-specific antigen (PSA), which can be used as a biomarker in studies, enhancing the cell line's utility in prostate cancer research.
GFP expression aids in the visualization of cell-cell interactions within the tumor microenvironment, particularly important in understanding the pathology of prostate cancer.
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