GFP Stable Cell Line-LNCAP

Name: GFP Stable Cell Line-LNCAP
SKU: CSC-RR0118
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RR0118

Host Cell : LNCaP Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Documents

Cat. No.	CSC-RR0118
Description	The LNCap cell line is a cell line derived from a needle human prostate adenocarcinoma cells of a 50-year old caucasian male in 1977, where cells were taken from a needle aspiration biopsy of a metastatic lesion in the left supraclavicular lymph node. The GFP Stable Cell Line-LNCAP constitutively expresses GFP.
Host Cell	LNCaP
Host Cell Species	Homo sapiens (Human)
Reporter Type	Fluorescent protein
Applications	1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Recommended Medium	Inquiry for instruction of culturing
Storage	Liquid nitrogen

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

CSC-RR0118-GFP Stable Cell Line-LNCAP-Datasheet

Quick Inquiry

Background

Case Study

Applications

Q & A

Customer Reviews

The LNCaP (Lymph Node Carcinoma of the Prostate) cell line is a human prostate cancer cell line that is commonly used to study prostate cancer biology and for drug development. The GFP stable cell line derived from LNCaP cells allows for the visualization of cell behavior, including cell proliferation, migration, and invasion, which are key processes in cancer progression.

The expression of GFP in LNCaP cells provides a convenient tool for studying the molecular mechanisms underlying prostate cancer development and for evaluating the efficacy of potential therapeutic agents. This stable cell line is particularly useful for in vitro and in vivo studies, as it allows researchers to track the behavior of prostate cancer cells in a living system, facilitating the understanding of disease progression and the development of targeted therapies.

The researchers discuss the different features of prostate cancer (PCa) cells dependent on PSA expression. PSA−/lo cells are resistant to stress, including androgen deprivation, have a high clonogenic capacity, and are tumorigenic over time. They express stem cell genes, divide asymmetrically to produce PSA+ cells, and initiate strong tumor growth in castrated animals. Importantly, they contain castration-resistant PCa cells with the ALDH+CD44+α2β1+ phenotype. PSA+ cells, on the other hand, have limited tumorigenicity, divide symmetrically, and are castration sensitive. This shows PSA−/lo cells are important donors of castration-resistant PCa cells.

Figure 1 depicts the significant differences in the molecular, cellular, and tumorigenic properties of prostate cancer cells with high PSA expression (PSA+) and low PSA expression/unstable expression (PSA−/lo). (doi: 10.1016/j.stem.2012.03.009) Figure 1. The researchers describe distinct properties of PCa cells expressing high (PSA+) and low (PSA−/lo) levels of PSA. PSA−/lo cells are quiescent, resistant to stress like androgen deprivation, highly clonogenic, and possess long-term tumorigenic capacity. They express stem cell genes, undergo asymmetric division generating PSA+ cells, initiate robust tumor growth, resist androgen ablation, and harbor highly tumorigenic castration-resistant PCa cells enriched via ALDH+CD44+α2β1+ phenotype. (Qin J, et al., 2021)

The GFP Stable Cell Line-LNCAP holds significant utility in diverse research domains owing to its unique characteristics. LNCAP cells, derived from human prostate adenocarcinoma, are widely utilized in prostate cancer research. The integration of the green fluorescent protein (GFP) into these cells enables researchers to track cellular processes, gene expression, and protein localization with exceptional precision.

(1)Live Cell Imaging of Prostate Cancer Progression: GFP Stable Cell Line-LNCAP allows for real-time monitoring of prostate cancer progression in vitro and in vivo. Researchers can observe tumor growth, metastasis, and interactions with the tumor microenvironment, providing valuable insights into disease mechanisms.

(2)Drug Screening and Development: Utilizing GFP Stable Cell Line-LNCAP in high-throughput drug screening assays enables the rapid evaluation of potential therapeutics for prostate cancer treatment. The GFP signal facilitates the quantification of cellular responses to drug candidates, aiding in the identification of novel anti-cancer agents.

(3)Studying Prostate Cancer Biology and Genetics: By employing GFP Stable Cell Line-LNCAP, researchers can investigate various aspects of prostate cancer biology, including signaling pathways, gene regulation, and cellular interactions. This cell line serves as a valuable tool for deciphering the molecular mechanisms underlying prostate cancer development and progression.

Customer Q&As

How does the expression of GFP in the GFP Stable Cell Line-LNCaP facilitate the study of prostate cancer cell interaction with the tumor microenvironment?

A: GFP fluorescence allows for the real-time visualization and tracking of LNCaP cells when co-cultured with other cell types or within a three-dimensional matrix, enabling the study of interactions with stromal cells, extracellular matrix components, and the impact of the microenvironment on prostate cancer cell behavior.

In what manner can the GFP Stable Cell Line-LNCaP be applied to assess the targeting accuracy of novel drug delivery systems designed for prostate cancer therapy?

A: Drug delivery systems can be labeled with a fluorescent tracer or conjugated to a compound that quenches GFP fluorescence upon interaction. The targeting accuracy can then be evaluated by fluorescence microscopy or flow cytometry, observing the colocalization or quenching of GFP in LNCaP cells.

Can the GFP Stable Cell Line-LNCaP be used to perform live-cell imaging to monitor cellular responses to androgen deprivation in real-time?

A: Yes, androgen deprivation effects on LNCaP cells can be monitored by live-cell imaging, using GFP fluorescence to observe changes in cell morphology, proliferation, and apoptosis over time, providing insights into cellular dynamics under hormone-deprived conditions.

How can the GFP Stable Cell Line-LNCaP aid in the identification and validation of cell surface markers unique to prostate cancer cells?

A: The stable GFP expression enables easy identification and sorting of LNCaP cells from a mixed population using flow cytometry. Subsequently, cell surface proteomics can be conducted to identify prostate cancer-specific markers, which can be validated by comparing GFP-positive cells with GFP-negative cells.

What experimental approaches could be employed using the GFP Stable Cell Line-LNCaP to investigate the mechanisms of metastasis in prostate cancer?

A: The cell line can be used in various assays, such as wound healing, transwell migration, and invasion assays, to study the mechanisms involved in prostate cancer metastasis. Additionally, intravital imaging in animal models can provide a visual assessment of metastatic behavior, tracking the migration of GFP-labeled LNCaP cells to distant sites.

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Customer Reviews

Hormone Sensitivity

This cell line is hormone-sensitive, reflecting key aspects of hormone-responsive prostate cancers, which is critical for testing hormonal therapies.

French

2020-07-09

Adherent Cell Line

The LNCAP cells adhere well to culture substrates, simplifying handling and enabling various in vitro assays without the need for suspension adaptation.

Canada

2020-09-25

Prostate Specific Antigen Expression

GFP Stable Cell Line-LNCAP expresses prostate-specific antigen (PSA), which can be used as a biomarker in studies, enhancing the cell line's utility in prostate cancer research.

French

2020-03-19

Visualizing Cell-Cell Interaction

GFP expression aids in the visualization of cell-cell interactions within the tumor microenvironment, particularly important in understanding the pathology of prostate cancer.

Germany

2020-11-23

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GFP Stable Cell Line-LNCAP

Cell Line Information

Cell Culture Information

Safety and Packaging

Documents

Background

Case Study

Applications

Q & A

Customer Reviews

How does the expression of GFP in the GFP Stable Cell Line-LNCaP facilitate the study of prostate cancer cell interaction with the tumor microenvironment?

In what manner can the GFP Stable Cell Line-LNCaP be applied to assess the targeting accuracy of novel drug delivery systems designed for prostate cancer therapy?

Can the GFP Stable Cell Line-LNCaP be used to perform live-cell imaging to monitor cellular responses to androgen deprivation in real-time?

How can the GFP Stable Cell Line-LNCaP aid in the identification and validation of cell surface markers unique to prostate cancer cells?

What experimental approaches could be employed using the GFP Stable Cell Line-LNCaP to investigate the mechanisms of metastasis in prostate cancer?

Hormone Sensitivity

Adherent Cell Line

Prostate Specific Antigen Expression

Visualizing Cell-Cell Interaction

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