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Luciferase Reporter Cell Line - DU145

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RR00972

Host Cell :   DU145 Size :   >1x106 frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RR00972
Description DU145-Luciferase cell line is engineered to express luciferase reporter gene in DU145 cells. Luciferase is a useful biomarker and is widely used in cell research to label and monitor various types of cells.This cell line is a powerful tool in bioluminescent tracking DU145 cells.
Product Type Stable cell line expressing luciferase reporter gene
Target Gene Luciferase
Host Cell DU145
Host Cell Species Homo sapiens (Human)
Applications 1) in vitro cell tracking by bioluminescent signal
2) monitor in vivo tumor growth using the bioluminescence signal
3) anticancer drug development
Size One vial of frozen cells, typically >1x10^6cells/vial
Stability This cell line is stable at least 10 passages.
Quality Control 1) in vitro cell luciferase assay
2) mycoplasma detection
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Adherent
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Target Gene Luciferase
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Perfect tool for prostate cancer pathway studies

We needed a reliable readout for androgen receptor signaling, and this cell line delivered outstanding performance. The luciferase expression remained robust over 15 passages without any detectable silencing. The assay window is wide, and the cells respond beautifully to our positive controls.

Germany

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