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Gene Mutagenesis Service


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Gene Mutagenesis Service

Frequently Asked Questions: Gene Mutagenesis Service

1.  What you need for our custom DNA template?

2.  What do site-directed mutagenesis service includes?

3.  What about the accuracy of mutated region?

4.  What can mutagenesis of existing genes help you?

5.  How long does the mutagenesis project need?

6.  What about our mutagenesis method?

7.  Can you achieve the mutations on large DNA constructs?


1. What do you need for our custom DNA template?

We need you to provide the starting materials including:

1)  Your DNA template plasmid as starting material. If the starting material is bacterial culture, please send the culture at enough amounts.

2)  The complete sequence of the template including the target gene and vector.

3)  Mutation specifications.

4)  Antibiotic resistance information of the template and destination vectors.

2. What does site-directed mutagenesis service include?

Our site-directed mutagenesis service includes 1) design, synthesis, and purification of mutagenic oligonucleotide primers; 2) PCR amplification; 3) transformation; 4) plasmid isolation; 5) sequence verification.

3. What about the accuracy of mutated region?

All mutated sequences will be verified with dsDNA Sequencing and a restriction digest for 100% accuracy.

4. What can mutagenesis of existing genes help you?

Mutagenesis of existing genes can help you accelerate your research, including:

1)  Investigate active sites, structure-function relationships, nucleic acid-protein interactions, etc.

    Test functional elements, such as promoter regions and terminator sequences, etc.

2)  Design knockout constructs;

3)  Construct tagged proteins and fusion proteins;

4)  Conduct alanine scans to identify protein functional domains;

5)  Generate alternative splice forms;

5. How long does the mutagenesis project need?

Our average turnaround time is 8-14 business days and could vary depending on the mutagenesis project.

6. What about our mutagenesis method?

We use PCR site-directed method combining with our proprietary protocols. Moreover, we sequence the mutated region through automated fluorescent sequencing

7. Can you achieve the mutations on large DNA constructs?

Our technology is optimized to introduce point mutations, deletion mutations, and insertion mutations on constructs as large as 12 kb, including target gene and its vector.

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