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Answer: The standard cloning vectors of Creative Biogene are pUC19 and pUC57; While, the standard expression vector is pcDNA 3.1 (+).
Answer: Yes, Creative Biogene can subclone the synthetic gene into the vector of your choice for a reasonable additional fee.
Answer: Each living species has a different preference for codon usage. The redundancy of the genetic code allows the exchange of rare codons for the most widely used codons encoding the same amino acid. For recombinant protein production purpose, this exchange permits a better expression of the protein in the chosen organism
Answer: Due to limitations in oligo synthesis chemistry and other practical considerations, oligos are produced as mixture of single-stranded DNA strands and are limited in length to ~200 bases. However, synthetic genes can extend to several thousand, double-stranded base pairs.
Answer: Creative Biogene will deliver 5.0ug plasmid DNA containing gene of interest in the vector and its bacterial stock.
Answer: There is no length limit. We routinely synthesize genes of 10 kb or longer using short DNA oligos as building blocks.
Answer: Yes. Creative Biogene conducts sequence verification during the gene synthesis process to ensure your synthesis product is as intended.