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Frequently Asked Questions: Gene Synthesis

  1. What is the standard vector does Creative Biogene clone into?
  2. Can Creative Biogene subclone the gene into the vector of my choice?
  3. What is codon optimization?
  4. What is the difference between a long oligo and a synthetic gene?
  5. How do you deliver synthetic genes?
  6. What gene length can Creative Biogene synthesize?
  7. Are the genes sequencing verified?

  1. What is the standard vector does Creative Biogene clone into?

    Answer: The standard cloning vectors of Creative Biogene are pUC19 and pUC57; While, the standard expression vector is pcDNA 3.1 (+).

  2. Can Creative Biogene subclone the gene into the vector of my choice?

    Answer: Yes, Creative Biogene can subclone the synthetic gene into the vector of your choice for a reasonable additional fee.

  3. What is codon optimization?

    Answer: Each living species has a different preference for codon usage. The redundancy of the genetic code allows the exchange of rare codons for the most widely used codons encoding the same amino acid. For recombinant protein production purpose, this exchange permits a better expression of the protein in the chosen organism

  4. What is the difference between a long oligo and a synthetic gene?

    Answer: Due to limitations in oligo synthesis chemistry and other practical considerations, oligos are produced as mixture of single-stranded DNA strands and are limited in length to ~200 bases. However, synthetic genes can extend to several thousand, double-stranded base pairs.

  5. How do you deliver synthetic genes?

    Answer: Creative Biogene will deliver 5.0ug plasmid DNA containing gene of interest in the vector and its bacterial stock.

  6. What gene length can Creative Biogene synthesize?

    Answer: There is no length limit. We routinely synthesize genes of 10 kb or longer using short DNA oligos as building blocks.

  7. Are the genes sequencing verified?

    Answer: Yes. Creative Biogene conducts sequence verification during the gene synthesis process to ensure your synthesis product is as intended.

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