Human TACR3-tGFP Stable Cell Line-SHSY5Y

Name: Human TACR3-tGFP Stable Cell Line-SHSY5Y
SKU: CSC-RG0030

Cat.No. : CSC-RG0030 Host Cell: SHSY5Y

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RG0030
Gene	TACR3
Gene Species	Homo sapiens (Human)
Alias	TACR3, NK3R, NKR, NK-3R, TAC3RL
Host Cell	SHSY5Y
Host Cell Species	Homo sapiens (Human)
Morphology	Epithelial
Stability	Validated for at least 10 passages
Application	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Shipping	Dry ice
Storage	Liquid nitrogen

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.
Growth Properties	Mixed, adherent and suspension

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Background

Publications

Q & A

Customer Reviews

Neurokinin 3 receptor is the gene that encodes a protein that is one of the three Neurokinin receptors (NKRs), also termed as TACRs. The Neurokinin receptor family is a group of Gcoupled receptors whose principal ligands are the Neurokinins. This protein family encompasses a wide range of functions including various parachine, autocrine and endocrine processes. NK3 receptors are distributed widely throughout the CNS, and are found in high levels in the cerebral cortex, basal ganglia and dorsal horn of the spinal chord. They have a limited distribution in peripheral tissues, and are found in ganglia (e.g., myenteric plexus), kidney, and in a limited number of smooth muscles (e.g., rat portal vein). NK3 antagonist has been proposed as antipsychotics.

Customer Q&As

What is the specific research focus or primary purpose of using the Human TACR3-tGFP Stable Cell Line-SHSY5Y in neuroscience or cell biology studies?

A: The Human TACR3-tGFP Stable Cell Line-SHSY5Y is used primarily to investigate the neurobiology of the Tachykinin Receptor 3 (TACR3) and its associated signaling pathways in SH-SY5Y neuroblastoma cells. It helps researchers understand receptor function and cellular responses in neuronal contexts.

Could you elaborate on the methods employed to generate and maintain the Human TACR3-tGFP Stable Cell Line-SHSY5Y, including any unique challenges in its maintenance?

A: Generating this stable cell line involves transfecting SH-SY5Y cells with a TACR3-tGFP fusion construct, followed by clonal selection and maintenance in selective media. Maintaining this cell line may require monitoring for changes in tGFP expression and ensuring proper culture conditions to retain stable expression over time.

What specific assays or experiments are commonly conducted using the Human TACR3-tGFP Stable Cell Line-SHSY5Y to study TACR3 receptor function and its downstream effects?

A: Researchers commonly employ the Human TACR3-tGFP Stable Cell Line-SHSY5Y in ligand binding studies, calcium imaging assays, and functional studies to elucidate TACR3-mediated cellular responses. It is also valuable for investigating receptor internalization dynamics upon ligand stimulation.

Are there any known differences in the TACR3 signaling pathways observed in the Human TACR3-tGFP Stable Cell Line-SHSY5Y compared to other cell lines or tissues?

A: The signaling pathways of TACR3 in the Human TACR3-tGFP Stable Cell Line-SHSY5Y may exhibit unique characteristics due to the cellular context of SH-SY5Y neuroblastoma cells. Researchers can study these specific signaling patterns to gain insights into TACR3 biology in neuronal settings.

Can you provide information on the potential applications of the Human TACR3-tGFP Stable Cell Line-SHSY5Y in drug discovery or therapeutic development for neurological disorders?

A: Investigating TACR3 using the Human TACR3-tGFP Stable Cell Line-SHSY5Y can contribute to drug discovery efforts targeting neurological disorders. It allows for the screening of compounds that modulate TACR3 activity, potentially leading to novel therapeutic interventions.

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