Human TACR1-tGFP Stable Cell Line-SHSY5Y

Name: Human TACR1-tGFP Stable Cell Line-SHSY5Y
SKU: CSC-RG0035
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0035

Host Cell : SH-SY5Y Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0035
Background	Neurokinin 1 receptor gene encodes the receptor for the tachykinin substance P, also referred to as Neurokinin 1. Neurokinin receptors family is a group of Gcoupled proteins whose principal ligands are Neurokinins. The NK1 receptor is localized in high concentrations in the CNS and it is thought to mediate central stress reactions, mood control, excitatory neurotransmission, etc. The binding of SP (Substance P) to the receptor seems to be implicated in the transmission of stress signals and pain.
Target Gene	TACR1
Gene Species	Homo sapiens (Human)
Abbr	SHSY5Y-HuTACR1-tGFP
Alias	TACR1, TAC1R, NK1R, NKIR, SPR, NK-1R
Host Cell	SH-SY5Y
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Mixed, adherent and suspension

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TACR1 tachykinin receptor 1 [ Homo sapiens ]
Gene Symbol	TACR1
Synonyms	TACR1; tachykinin receptor 1; TAC1R; substance-P receptor; NK1R; NKIR; SPR; NK-1R; NK-1 receptor; tachykinin receptor 1 (substance P receptor; neurokinin-1 receptor);
GeneID	6869
Uni ProtID	P25103
Chromosome Location	2p13.1-p12
Function	G-protein coupled receptor activity; protein binding; receptor activity; signal transducer activity; substance P receptor activity; tachykinin receptor activity;
Pathway	Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; Measles, organism-specific biosystem;
MIM	162323

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Customer Q&As

How is the Human TACR1-tGFP Stable Cell Line-SHSY5Y achieved through genetic engineering?

A: The Human TACR1-tGFP Stable Cell Line-SHSY5Y is achieved by fusing the TACR1 (T cell activation regulator 1) gene with the tGFP (turbo green fluorescent protein) gene and then transfecting this fusion gene into the SHSY5Y cell line. This fusion gene allows researchers to observe the expression and distribution of TACR1 within cells, as the fluorescent properties of tGFP cause the cells to emit green fluorescence when illuminated with specific wavelengths of light.

The Human TACR1-tGFP Stable Cell Line-SHSY5Y is achieved by fusing the TACR1 (T cell activation regulator 1) gene with the tGFP (turbo green fluorescent protein) gene and then transfecting this fusion gene into the SHSY5Y cell line. This fusion gene allows researchers to observe the expression and distribution of TACR1 within cells, as the fluorescent properties of tGFP cause the cells to emit green fluorescence when illuminated with specific wavelengths of light.

A: This cell line serves as a model system for studying the dynamic changes of TACR1 during the process of T cell activation. By observing the fluorescence of tGFP, researchers can track the localization and movement of TACR1 within cells, which aids in understanding the function of TACR1 in T cell activation and immune responses.

Does the expression of tGFP in the Human TACR1-tGFP Stable Cell Line-SHSY5Y affect the normal function of the cells?

A: Typically, the expression of tGFP has a minimal impact on the normal function of cells. However, if the expression level of tGFP is too high, it may burden the cell's energy metabolism or affect the folding and function of other proteins within the cell.

What is the application of the Human TACR1-tGFP Stable Cell Line-SHSY5Y in cell differentiation research?

A: This cell line can be used to study the role of TACR1 in the process of cell differentiation. By observing the expression pattern of tGFP, researchers can understand the regulatory mechanisms of TACR1 during cell maturation and differentiation.

What is the application of the Human TACR1-tGFP Stable Cell Line-SHSY5Y in the study of immune cell development?

A: This cell line can be used to study the role of TACR1 in the development of immune cells. By observing the expression and distribution of tGFP, researchers can understand the key role of TACR1 in the formation of immune cell functions.

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