Cat.No. : CSC-RG0035 Host Cell: SHSY5Y
| Cat. No. | CSC-RG0035 |
| Background | Neurokinin 1 receptor gene encodes the receptor for the tachykinin substance P, also referred to as Neurokinin 1. Neurokinin receptors family is a group of Gcoupled proteins whose principal ligands are Neurokinins. The NK1 receptor is localized in high concentrations in the CNS and it is thought to mediate central stress reactions, mood control, excitatory neurotransmission, etc. The binding of SP (Substance P) to the receptor seems to be implicated in the transmission of stress signals and pain. |
| Gene | TACR1 |
| Gene Species | Homo sapiens (Human) |
| Alias | TACR1, TAC1R, NK1R, NKIR, SPR, NK-1R |
| Host Cell | SHSY5Y |
| Host Cell Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Mixed, adherent and suspension |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human TACR1-tGFP Stable Cell Line-SHSY5Y is achieved by fusing the TACR1 (T cell activation regulator 1) gene with the tGFP (turbo green fluorescent protein) gene and then transfecting this fusion gene into the SHSY5Y cell line. This fusion gene allows researchers to observe the expression and distribution of TACR1 within cells, as the fluorescent properties of tGFP cause the cells to emit green fluorescence when illuminated with specific wavelengths of light.
A: This cell line serves as a model system for studying the dynamic changes of TACR1 during the process of T cell activation. By observing the fluorescence of tGFP, researchers can track the localization and movement of TACR1 within cells, which aids in understanding the function of TACR1 in T cell activation and immune responses.
A: Typically, the expression of tGFP has a minimal impact on the normal function of cells. However, if the expression level of tGFP is too high, it may burden the cell's energy metabolism or affect the folding and function of other proteins within the cell.
A: This cell line can be used to study the role of TACR1 in the process of cell differentiation. By observing the expression pattern of tGFP, researchers can understand the regulatory mechanisms of TACR1 during cell maturation and differentiation.
A: This cell line can be used to study the role of TACR1 in the development of immune cells. By observing the expression and distribution of tGFP, researchers can understand the key role of TACR1 in the formation of immune cell functions.
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