Human DRD5-tGFP Stable Cell Line-SHSY5Y

Name: Human DRD5-tGFP Stable Cell Line-SHSY5Y
SKU: CSC-RG0038
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0038

Host Cell : SH-SY5Y Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0038
Background	The gene encodes the D5 subtype of the Dopamine receptor. This subtype is a G-protein coupled receptor which stimulates adenylyl cyclase. Dopamine is one of the most important neurotransmitters and the expression of its receptors is well characterized in brain. Dopamine receptors are involved in many neurological processes so their abnormal signalling is implicated in several neuropsychiatric disorders. Defects in DRD5 are a cause of benign essential blepharospasm (BEB), a primary focal dystonia affecting the orbicularis oculi muscles.
Target Gene	DRD5
Gene Species	Homo sapiens (Human)
Abbr	SHSY5Y-HuDRD5-tGFP
Alias	DRD5, DRD1L2, DRD1B, DBDR
Host Cell	SH-SY5Y
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Mixed, adherent and suspension

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	DRD5 dopamine receptor D5 [ Homo sapiens ]
Gene Symbol	DRD5
Synonyms	DRD5; dopamine receptor D5; DRD1L2; D(1B) dopamine receptor; DRD1B; dopamine D5 receptor; dopamine receptor D1B; d dopamine receptor; D1beta dopamine receptor; DBDR; MGC10601;
GeneID	1816
Uni ProtID	P21918
mRNA Refseq	BC009748
Chromosome Location	4p16.1
Function	G-protein alpha-subunit binding; G-protein coupled receptor activity; dopamine binding; dopamine receptor activity; dopamine receptor activity, coupled via Gs; drug binding; receptor activity; signal transducer activity;
Pathway	Amine ligand-binding receptors, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Dopamine receptors, organism-specific biosystem; Dopaminergic synapse, organism-specific biosystem; Dopaminergic synapse, conserved biosystem;

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How does the Human DRD5-tGFP Stable Cell Line-SHSY5Y facilitate the study of dopamine receptor function in neurological disorders?

A: The Human DRD5-tGFP Stable Cell Line-SHSY5Y is engineered to express the human dopamine receptor D5 (DRD5) tagged with green fluorescent protein (tGFP). This allows researchers to visualize and quantify the activity of DRD5 receptors in response to dopamine or other ligands, which is crucial for understanding the role of these receptors in the pathophysiology of neurological disorders such as schizophrenia and Parkinson's disease.

What are the advantages of using the Human DRD5-tGFP Stable Cell Line-SHSY5Y for drug screening compared to traditional animal models?

A: The Human DRD5-tGFP Stable Cell Line-SHSY5Y provides a more physiologically relevant and ethically acceptable platform for drug screening. It allows for high-throughput screening of potential therapeutic compounds, reducing the reliance on animal testing. Additionally, it offers the advantage of being able to directly observe the effects of drugs on human receptors, which can lead to more accurate predictions of drug efficacy and safety in humans.

How does the Human DRD5-tGFP Stable Cell Line-SHSY5Y contribute to the understanding of dopamine receptor pharmacology?

A: This cell line enables researchers to study the pharmacological properties of DRD5 receptors, such as their affinity for various ligands and their response to different agonists and antagonists. By observing the fluorescent signal, researchers can gain insights into the receptor's activation and desensitization processes, which are essential for developing new drugs targeting these receptors.

How does the Human DRD5-tGFP Stable Cell Line-SHSY5Y aid in the development of novel therapeutic strategies for dopamine-related disorders?

A: By providing a platform to study the DRD5 receptor's function and response to various compounds, this cell line can accelerate the discovery of new drugs and therapeutic interventions. It can also be used to test the efficacy and safety of existing drugs, potentially leading to new indications or improved treatment protocols.

How does the Human DRD5-tGFP Stable Cell Line-SHSY5Y help in understanding the molecular mechanisms of DRD5 receptor signaling?

A: The cell line allows researchers to investigate the intracellular signaling pathways activated by the DRD5 receptor. By monitoring the fluorescent signal, they can trace the activation of downstream signaling molecules, which can lead to a better understanding of the molecular mechanisms underlying the receptor's function.

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