Cat.No. : CSC-RG0038 Host Cell: SHSY5Y
| Cat. No. | CSC-RG0038 |
| Background | The gene encodes the D5 subtype of the Dopamine receptor. This subtype is a G-protein coupled receptor which stimulates adenylyl cyclase. Dopamine is one of the most important neurotransmitters and the expression of its receptors is well characterized in brain. Dopamine receptors are involved in many neurological processes so their abnormal signalling is implicated in several neuropsychiatric disorders. Defects in DRD5 are a cause of benign essential blepharospasm (BEB), a primary focal dystonia affecting the orbicularis oculi muscles. |
| Gene | DRD5 |
| Gene Species | Homo sapiens (Human) |
| Alias | DRD5, DRD1L2, DRD1B, DBDR |
| Host Cell | SHSY5Y |
| Host Cell Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Mixed, adherent and suspension |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human DRD5-tGFP Stable Cell Line-SHSY5Y is engineered to express the human dopamine receptor D5 (DRD5) tagged with green fluorescent protein (tGFP). This allows researchers to visualize and quantify the activity of DRD5 receptors in response to dopamine or other ligands, which is crucial for understanding the role of these receptors in the pathophysiology of neurological disorders such as schizophrenia and Parkinson's disease.
A: The Human DRD5-tGFP Stable Cell Line-SHSY5Y provides a more physiologically relevant and ethically acceptable platform for drug screening. It allows for high-throughput screening of potential therapeutic compounds, reducing the reliance on animal testing. Additionally, it offers the advantage of being able to directly observe the effects of drugs on human receptors, which can lead to more accurate predictions of drug efficacy and safety in humans.
A: This cell line enables researchers to study the pharmacological properties of DRD5 receptors, such as their affinity for various ligands and their response to different agonists and antagonists. By observing the fluorescent signal, researchers can gain insights into the receptor's activation and desensitization processes, which are essential for developing new drugs targeting these receptors.
A: By providing a platform to study the DRD5 receptor's function and response to various compounds, this cell line can accelerate the discovery of new drugs and therapeutic interventions. It can also be used to test the efficacy and safety of existing drugs, potentially leading to new indications or improved treatment protocols.
A: The cell line allows researchers to investigate the intracellular signaling pathways activated by the DRD5 receptor. By monitoring the fluorescent signal, they can trace the activation of downstream signaling molecules, which can lead to a better understanding of the molecular mechanisms underlying the receptor's function.
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