HumanDRD3-tGFP Stable Cell Line-SHSY5Y

Name: HumanDRD3-tGFP Stable Cell Line-SHSY5Y
SKU: CSC-RG0037
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0037

Host Cell : SH-SY5Y Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0037
Background	The gene encodes the D3 subtype of the Dopamine receptor. This subtype is a G-protein coupled receptor which stimulates adenylyl cyclase. Dopamine is one of the most important neurotransmitters and the expression of its receptors is well characterized in brain. Dopamine receptors are involved in many neurological processes so their abnormal signalling is implicated in several neuropsychiatric disorders. DRD3 is found in the limbic areas of the brain, which usually are associated with cognitive, emotional, and endocrine functions. DRD3 is a target for drugs which treat schizophrenia, drug addiction, and Parkinson"s disease.
Target Gene	DRD3
Gene Species	Homo sapiens (Human)
Abbr	SHSY5Y-HuDRD3-tGFP
Alias	DRD3, D3DR, ETM1, FET1
Host Cell	SH-SY5Y
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Mixed, adherent and suspension

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	DRD3 dopamine receptor D3 [ Homo sapiens ]
Gene Symbol	DRD3
Synonyms	D3DR; ETM1; FET1
Gene Description	dopamine receptor D3
GeneID	1814
Uni ProtID	P35462
mRNA Refseq	NM_000796.3
Protein Refseq	NP_000787.2
Chromosome Location	3q13.3
Function	dopamine binding; dopamine receptor activity, coupled via Gi/Go; dopamine receptor activity, coupled via Gi/Go; drug binding; drug binding; protein binding; protein domain specific binding;
Pathway	Amine ligand-binding receptors, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Dopamine receptors, organism-specific biosystem; Dopaminergic synapse, organism-specific biosystem; Dopaminergic synapse, conserved biosystem; G alpha (i) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem;
MIM	126451

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Customer Reviews

Customer Q&As

What specific role does the dopamine D3 receptor (DRD3) play in the neuronal development and function of the Human DRD3-tGFP Stable Cell Line-SHSY5Y?

A: The dopamine D3 receptor (DRD3) in the Human DRD3-tGFP Stable Cell Line-SHSY5Y is crucial for modulating neuronal activity, particularly in the context of reward, motivation, and motor control. This receptor is involved in the regulation of dopaminergic signaling, which is essential for the development and function of neurons in the brain. The tGFP (tetracycline-inducible green fluorescent protein) tag allows for the visualization of DRD3 expression, aiding in the study of receptor localization and activity within the cells.

How does the Human DRD3-tGFP Stable Cell Line-SHSY5Y model contribute to the understanding of dopamine-related disorders?

A: The Human DRD3-tGFP Stable Cell Line-SHSY5Y serves as a valuable model for studying dopamine-related disorders, such as Parkinson's disease and schizophrenia, where DRD3 dysfunction is implicated. By manipulating the expression of DRD3 in these cells, researchers can investigate the molecular mechanisms underlying these conditions and test potential therapeutic interventions.

How does the Human DRD3-tGFP Stable Cell Line-SHSY5Y facilitate the study of drug interactions with the DRD3 receptor?

A: The Human DRD3-tGFP Stable Cell Line-SHSY5Y provides a platform for high-throughput screening of compounds that interact with the DRD3 receptor. The fluorescent tag allows for the direct visualization of receptor activation, enabling researchers to assess the efficacy and selectivity of potential drugs in real-time.

How does the Human DRD3-tGFP Stable Cell Line-SHSY5Y differ from primary neuronal cultures in terms of studying DRD3 function?

A: The Human DRD3-tGFP Stable Cell Line-SHSY5Y offers a more controlled environment for studying DRD3 function compared to primary neuronal cultures. It allows for consistent genetic backgrounds and experimental conditions, which can be challenging to achieve with primary cultures. However, primary cultures may provide a more complex and physiologically relevant context for DRD3 function.

What are the potential applications of the Human DRD3-tGFP Stable Cell Line-SHSY5Y in drug discovery and development?

A: The Human DRD3-tGFP Stable Cell Line-SHSY5Y can be used to screen for compounds that modulate DRD3 activity, aiding in the development of new drugs for conditions related to dopamine dysregulation. It can also be used to study the pharmacokinetics and pharmacodynamics of potential therapeutics.

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