Cat.No. : CSC-RG0036 Host Cell: SHSY5Y
| Cat. No. | CSC-RG0036 |
| Background | The gene encodes the D1 subtype of the Dopamine receptor. This subtype is a G-protein coupled receptor which stimulates adenylyl cyclase. Dopamine is one of the most important neurotransmitters and the expression of its receptors is well characterized in brain. Dopamine receptors are involved in many neurological processes so their abnormal signalling is implicated in several neuropsychiatric disorders. DRD1 mediates some behavioural responses, regulates neuronal growth and development. |
| Gene | DRD1 |
| Gene Species | Homo sapiens (Human) |
| Alias | DRD1, DADR, DRD1A |
| Host Cell | SHSY5Y |
| Host Cell Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Mixed, adherent and suspension |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human DRD1-tGFP Stable Cell Line-SHSY5Y is primarily used for investigating the dopamine receptor D1 (DRD1) and its associated signaling pathways, providing insights into neurobiology and neuropharmacology.
A: The SHSY5Y cell line is derived from human neuroblastoma and is commonly used in neuroscience research. It was selected for creating the Human DRD1-tGFP Stable Cell Line due to its neuronal origin, making it a suitable model for studying dopamine receptors and neuronal signaling pathways.
A: The tGFP reporter allows for the visualization and tracking of the DRD1 receptor in live cells, enabling researchers to study its subcellular localization and dynamics in response to various stimuli or drugs.
A: This cell line is instrumental in elucidating DRD1 biology by enabling researchers to study receptor localization, trafficking, and responses to various ligands or drugs. Findings can have implications for developing novel therapies targeting DRD1 in neurologic and psychiatric disorders.
A: While the primary purpose of the Human DRD1-tGFP Stable Cell Line-SHSY5Y is DRD1 research, it can potentially be modified for other applications by replacing the DRD1 gene with other genes of interest, provided proper controls and modifications are implemented.
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