HumanDRD1-tGFP Stable Cell Line-SHSY5Y

Name: HumanDRD1-tGFP Stable Cell Line-SHSY5Y
SKU: CSC-RG0036
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0036

Host Cell : SH-SY5Y Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0036
Background	The gene encodes the D1 subtype of the Dopamine receptor. This subtype is a G-protein coupled receptor which stimulates adenylyl cyclase. Dopamine is one of the most important neurotransmitters and the expression of its receptors is well characterized in brain. Dopamine receptors are involved in many neurological processes so their abnormal signalling is implicated in several neuropsychiatric disorders. DRD1 mediates some behavioural responses, regulates neuronal growth and development.
Target Gene	DRD1
Gene Species	Homo sapiens (Human)
Abbr	SHSY5Y-HuDRD1-tGFP
Alias	DRD1, DADR, DRD1A
Host Cell	SH-SY5Y
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Mixed, adherent and suspension

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	DRD1 dopamine receptor D1 [ Homo sapiens ]
Gene Symbol	DRD1
Synonyms	DADR; DRD1A
Gene Description	dopamine receptor D1
GeneID	13488
Uni ProtID	P21728
mRNA Refseq	NM_000794.3
Protein Refseq	NP_000785.1
Chromosome Location	5q35.1
Function	dopamine binding; dopamine receptor activity; dopamine receptor activity, coupled via Gs; dopamine receptor activity, coupled via Gs; drug binding; protein binding;
Pathway	Alcoholism, organism-specific biosystem; Alcoholism, conserved biosystem; Amine ligand-binding receptors, organism-specific biosystem; Amphetamine addiction, organism-specific biosystem; Amphetamine addiction, conserved biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem;
MIM	126449

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What is the primary research focus or application of the Human DRD1-tGFP Stable Cell Line-SHSY5Y in the field of neuroscience or cell biology?

A: The Human DRD1-tGFP Stable Cell Line-SHSY5Y is primarily used for investigating the dopamine receptor D1 (DRD1) and its associated signaling pathways, providing insights into neurobiology and neuropharmacology.

How was the SHSY5Y cell line chosen as the host for creating the Human DRD1-tGFP Stable Cell Line, and what are its advantages in this context?

A: The SHSY5Y cell line is derived from human neuroblastoma and is commonly used in neuroscience research. It was selected for creating the Human DRD1-tGFP Stable Cell Line due to its neuronal origin, making it a suitable model for studying dopamine receptors and neuronal signaling pathways.

How does the tGFP reporter in the Human DRD1-tGFP Stable Cell Line aid in the study of dopamine receptor D1?

A: The tGFP reporter allows for the visualization and tracking of the DRD1 receptor in live cells, enabling researchers to study its subcellular localization and dynamics in response to various stimuli or drugs.

How can the Human DRD1-tGFP Stable Cell Line-SHSY5Y contribute to the understanding of dopamine receptor D1 biology and potential implications for drug development?

A: This cell line is instrumental in elucidating DRD1 biology by enabling researchers to study receptor localization, trafficking, and responses to various ligands or drugs. Findings can have implications for developing novel therapies targeting DRD1 in neurologic and psychiatric disorders.

Can this cell line be adapted or modified for additional experimental applications beyond DRD1 research?

A: While the primary purpose of the Human DRD1-tGFP Stable Cell Line-SHSY5Y is DRD1 research, it can potentially be modified for other applications by replacing the DRD1 gene with other genes of interest, provided proper controls and modifications are implemented.

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