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Yeast two-hybrid (Y2H) technology is a cornerstone method for probing protein–protein interactions directly within living cells. From its classical nuclear format to membrane-based, cytosolic, split-protein, and sequencing-enabled variants, Y2H has continuously evolved to address increasingly complex biological questions. These methodological advances enable the analysis of diverse protein classes and support scalable interaction discovery, from focused validation to systems-level interactome mapping. Today, Y2H remains a versatile and powerful platform for elucidating cellular mechanisms, signaling networks, and disease-associated protein interactions.
In the past few years, therapeutic microRNA (miRNA) is some of the most important biopharmaceuticals that are in commercial space as future medicines. The discovery of therapeutic miRNA is considered one of the most exhilarating and significant therapeutic breakthroughs in pharmaceutical development.
Over the past few decades, RNA sequencing has significantly progressed, becoming a paramount approach for transcriptome profiling.
RNA interference (RNAi), a process that involves sequence-specific gene silencing, is an endogenous post-transcriptional regulation process that consists of non-coding RNAs like microRNAs (miRNAs), small interfering RNAs (siRNAs), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs)...
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) system is widely used as a genome-editing tool in various organisms, including plants, to elucidate the fundamental understanding of gene function, disease diagnostics, and crop improvement.
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