LAPC-4 Cell Line

Brief Introduction
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	LAPC-4 (Los Angeles Prostate Cancer-4) is a human prostate cancer cell line established by K.A. Klein, R.E. Reiter, and their colleagues in 1997. The cell line was derived from a lymph node metastasis in a male patient with refractory prostate cancer. LAPC-4 was initially propagated as a patient-derived xenograft (PDX) model in male severe combined immunodeficient (SCID) mice before being adapted for in vitro cell culture. LAPC-4 cells grow as an adherent monolayer and exhibit an epithelioid morphology. In the field of prostate cancer translational research, LAPC-4 is recognized as a rare and highly valuable model because it endogenously expresses wild-type androgen receptor (AR), wild-type p53, and wild-type PTEN. This makes LAPC-4 an indispensable model for studying physiological androgen responses, the transition of prostate cancer from a hormone-sensitive state to castration-resistant prostate cancer (CRPC), and the biological characteristics of prostate-specific membrane antigen (PSMA).
Tissue	Prostate; derived from metastatic site (Lymph node)
Disease	Prostate Adenocarcinoma / Hormone-Sensitive to Castration-Resistant Prostate Cancer
Morphology	Epithelial-like
Gender	Male
Age	Unspecified
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 (Biosafety classification aligns with standard international guidelines for non-infectious human tumor cell lines)
Applications	1. Investigating "gold-standard" in vitro models for studying wild-type androgen receptor (AR) signaling mechanisms and transcriptional activation; 2. Exploring the mechanisms underlying androgen deprivation, the development of castration resistance, and resistance to anti-androgen drugs (e.g., enzalutamide, abiraterone); 3. Elucidating the expression and intracellular trafficking of prostate-specific membrane antigen (PSMA/FOLH1), as well as PSMA-targeted radionuclide or antibody therapies; 4. Characterizing the kinetics of endogenous prostate-specific antigen (PSA/KLK3) synthesis and secretion under physiological androgen stimulation; 5. Establishing subcutaneous or orthotopic xenograft models (including PDX and cell line-derived models) in immunodeficient rodent models.
Shipped In	Dry ice
Storage Temperature	−196°C
Characteristics
Tumorigenic	Yes, highly tumorigenic; forms androgen-dependent or castration-resistant tumors when engrafted into male SCID or nude mice.
Transformant	None; immortalized spontaneously from clinical metastatic tumor tissue via serial xenograft passage.
Receptor Profile	Wild-type androgen receptor (AR wt). Unlike LNCaP cells, which harbor a mutant AR, LAPC-4 cells retain an unmutated wild-type AR that responds physiologically to dihydrotestosterone (DHT) and the synthetic androgen R1881.
Expression Profile	Capable of naturally synthesizing and secreting prostate-specific antigen (PSA), while simultaneously overexpressing prostate-specific membrane antigen (PSMA) and prostate stem cell antigen (PSCA).
Growth Kinetics	Slow to moderate adherent expansion; typical population doubling time spans approximately 40 to 72 hours. Cells frequently display delicate attachment kinetics post-thaw.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Completely aspirate and discard the old complete culture medium from the culture vessel. 2. Gently wash the cell monolayer with sterile PBS (without Ca2+/Mg2+) to remove residual serum containing trypsin inhibitors. 3. Add 1.0 to 2.0 mL of 0.25% trypsin-0.53 mM EDTA (or Accutase) solution, pre-warmed to the appropriate temperature, ensuring the cell layer is completely covered. 4. Incubate at 37°C for 3 to 6 minutes; monitor under an inverted microscope until the cells round up and detach. 5. Add an equal volume of pre-warmed complete growth medium to immediately stop the enzymatic activity. 6. Gently pipette the suspension to disperse cell clumps, centrifuge at approximately 125 × g for 5 minutes, discard the supernatant, gently resuspend the cells in fresh complete growth medium, and distribute them into new culture vessels.
Thawing Protocol Note	LAPC-4 cells are exceptionally sensitive to ambient pH fluctuations and osmotic stress during early post-thaw recovery. Prior to introducing the thawed vial contents, place the culture vessel containing fresh complete growth medium into the 5% CO2 incubator for at least 15 minutes to allow the medium to equilibrate to its physiological pH range (7.0 to 7.6).
Medium Renewal	2 to 3 times per week
Subcultivation Ratio	Split confluent cultures (70-80% density) at a standard ratio of 1:2 to 1:4.
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	90% Complete culture growth medium + 10% DMSO (or 50% IMDM + 40% FBS + 10% DMSO)

Cat.No.	Product Name	Price
CSC-RR01205	FLuc Reporter Cell Line - LAPC-4	Inquiry
CSC-RR01206	Nluc Reporter Cell Line - LAPC-4	Inquiry

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* For research use only. Not intended for any clinical use.

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