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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | DU4475 is a human breast cancer cell line derived from a cutaneous metastasis in a 70-year-old female patient with advanced breast cancer. The cell line exhibits a lymphoblast-like or rounded morphology and grows primarily in suspension or as loosely adherent cell clusters—characteristics relatively uncommon among breast cancer cell lines. Phenotypically, DU4475 is classified as a triple-negative breast cancer (TNBC) model, as it lacks expression of the estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2). Genetically, the cell line displays an aneuploid karyotype and harbors mutations in key cancer-associated genes, such as BRAF (V600E mutation) and TP53. Due to its unique growth characteristics, TNBC marker profile, and BRAF-mutant genetic background, DU4475 has become a valuable in vitro model for investigating TNBC biology, exploring BRAF signaling dynamics, evaluating kinase-targeted inhibitors, and screening candidate anticancer drugs. |
| Tissue | Breast; derived from metastatic site (Skin / Cutaneous metastasis) |
| Disease | Breast Carcinoma; Triple-Negative Breast Cancer (TNBC) |
| Morphology | Lymphoblast-like / Rounded |
| Gender | Female |
| Age | 70 years |
| Product Format | Frozen |
| Growth Mode | Suspension |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Research on the biological characteristics and metastatic progression of triple-negative breast cancer (TNBC); 2. Evaluation of targeted therapies, particularly inhibitors addressing the BRAF V600E mutation and the MEK/ERK signaling pathway; 3. Investigation of anchorage-independent growth mechanisms and signal transduction in Luminal and Basal breast cancer subtypes; 4. High-throughput screening of candidate drugs and functional genomic analysis. |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Karyotype | Aneuploid |
| Tumorigenic | Yes, tumorigenic in immunocompromised mice |
| Genetic Profile | BRAF V600E mutation; TP53 mutation; ER negative (ER-); PR negative (PR-); HER2 negative (HER2-) |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Maintain the culture by adding fresh medium or replacing the medium. 2. Establish the culture by centrifuging (1,000 rpm, 5 minutes) and resuspending the cells (at a density of 2 × 10⁵ to 4 × 10⁵ viable cells/mL). 3. Do not allow the cell concentration to exceed 1 × 10⁶ cells/mL. 4. Gently resuspend the cells to prepare a single-cell suspension before subculturing into a new culture vessel. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | Maintain cell density between 2 × 10⁵ and 1 × 10⁶ cells/mL (split ratio approximately 1:2 to 1:4) |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO0160 | Cas9 Stable Cell Line - DU4475 | Inquiry |
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