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| General Information | |
| Organism | Hybrid (Rattus norvegicus X Mus musculus, Rat X Mouse) |
| Cell Line Description | VSC4.1 is a conditionally immortalized hybrid motor neuron-like cell line. This cell line was established using somatic cell fusion technology: specifically, by fusing neurons isolated from the ventral spinal cord (anterior horn region) of rat embryos with the HGPRT-deficient mouse neuroblastoma cell line N18TG2. The resulting somatic cell hybrid not only successfully retains the key biochemical, physiological, and morphological characteristics of lower motor neurons but also fully leverages the robust proliferative capacity and extended lifespan conferred by its neuroblastoma parent. VSC4.1 is widely utilized in research investigating the mechanisms of neurodegenerative diseases—particularly in fields such as Amyotrophic Lateral Sclerosis (ALS), Spinal Cord Injury (SCI), and calcium-dependent excitotoxicity. Furthermore, it is extensively employed for the screening of novel neuroprotective compounds. |
| Tissue | Spinal Cord (Ventral Horn) / Neural Crest |
| Cell Type | Motor Neuron-like Hybridoma |
| Disease | None (Derived from normal embryonic spinal cord tissue fused with a neuroblastoma line) |
| Morphology | Adherent polygonal cells in an undifferentiated state; transitions to a highly arborized neuronal phenotype with long, branching neurites upon differentiation. |
| Gender | Unspecified |
| Age | Embryonic (Rat donor tissue) |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Development of in vitro models for studying the physiological function, axonal elongation, and structural degeneration of lower motor neurons; 2. Investigation into the mechanisms of pathological biological cascades in Motor Neuron Disease (MND), including Amyotrophic Lateral Sclerosis (ALS); 3. Elucidation of the mechanisms underlying calcium ion (Ca²⁺) dysregulation and immunoglobulin-mediated cytotoxicity in ALS; 4. Preclinical screening of neuroprotective small molecules, antioxidants, and anti-apoptotic agents; 5. Modeling of neuroinflammatory injury pathways driven by microglial activation and cytokine exposure. |
| Shipped In | Dry ice |
| Storage Temperature | −196°C (Liquid nitrogen vapor phase) |
| Characteristics | |
| Tumorigenic | Yes, retains proliferative and tumorigenic potential under non-differentiated baseline states due to the neuroblastoma fusion partner. |
| Origin Hybridization | Somatic fusion product combining Rat Ventral Spinal Cord cells and Mouse N18TG2 Neuroblastoma cells. |
| Lineage Markers | It stains positively for standard neuronal cytoskeletal components, including neurofilament-H (NF-H), synaptophysin, and neuron-specific enolase (NSE). It exhibits a distinct cholinergic phenotype, expressing choline acetyltransferase (ChAT). |
| Growth Kinetics | Rapid adherent proliferation during the undifferentiated growth state; typical doubling time spans 24 to 36 hours. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing (Expansion) | 1. Remove and discard the spent culture medium from the culture flask. 2. Gently rinse the cell monolayer with sterile, Ca²⁺/Mg²⁺-free PBS to remove residual serum components containing trypsin inhibitors. 3. Add 1.0 to 2.0 mL of 0.25% Trypsin–0.53 mM EDTA solution to cover the cell layer. 4. Incubate at 37°C for approximately 3 minutes; observe under an inverted microscope until 70%–80% of the cells have rounded up and detached. Gently tap the culture flask to ensure complete detachment of the cells. 5. Add an equal volume of complete culture medium to thoroughly neutralize the trypsin activity. 6. Centrifuge the cell suspension at 300-500 × g for 5 minutes; carefully aspirate the supernatant, gently resuspend the cell pellet in fresh complete growth medium, and then seed into a new culture vessel. |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is standardly recommended for routine maintenance. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | 90% Complete growth medium + 10% DMSO (or 95% Complete medium + 5% DMSO) |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO02580 | Cas9 Stable Cell Line - VSC4.1 | Inquiry |
| CSC-RR00883 | GFP Reporter Cell Line - VSC4.1 | Inquiry |
| CSC-RR00932 | Luciferase Reporter Cell Line - VSC4.1 | Inquiry |
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