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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | JeKo-1 is a human mantle cell lymphoma (MCL) cell line established in 1997, derived from the peripheral blood mononuclear cells of a 78-year-old female patient diagnosed with MCL (leukemic variant). This cell line was isolated after the patient had undergone multiple rounds of chemotherapy and her disease had entered a refractory relapse phase. Distinguished by its hallmark genomic translocation t(11;14)(q13;q32)—a translocation that drives the constitutive overexpression of Cyclin D1 (CCND1)—JeKo-1 is globally recognized as the "gold standard" in vitro model for the study of mantle cell lymphoma. JeKo-1 is widely classified as a representative, high-grade cell line exhibiting aggressive characteristics within the spectrum of MCL. It serves as an indispensable tool for investigating cell cycle dysregulation, BCR-mediated signaling networks, mechanisms of resistance to BTK inhibitors, and for screening novel therapeutic targets in B-cell malignancies. |
| Tissue | Peripheral Blood |
| Disease | Mantle Cell Lymphoma (MCL; Leukemic Variant) |
| Morphology | Lymphoblast-like; round/oval floating single cells and loose aggregates |
| Gender | Female |
| Age | 78 years |
| Product Format | Frozen |
| Growth Mode | Suspension |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Molecular pathogenic mechanisms of t(11;14)-positive and cyclin D1-overexpressing mantle cell lymphoma 2. Elucidation of B-cell receptor (BCR) signaling architecture and downstream pathways 3. Characterization of mechanisms underlying sensitivity or acquired resistance to BTK inhibitors 4. Preclinical validation of targeted therapies (e.g., BCL2 inhibitors, CDK4/6 inhibitors) 5. Establishment of systemic and subcutaneous xenograft animal models for MCL |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, highly tumorigenic in immunocompromised mice (e.g., SCID or NSG mice) |
| Karyotype | Human hyperdiploid karyotype; modal number = 47/48; contains structural rearrangements including: t(11;14)(q13.3;q32.33), der(2)t(2;8)(p11.2;q24.2), der(17)t(13;17)(q14.1;p13), and add(22)(q11.2). |
| Genetic Profile | 1. Translocation: The characteristic t(11;14) translocation, which rearranges the CCND1 locus to the IGH enhancer region, resulting in the overexpression of Cyclin D1. 2. Mutations: TP53 mutations; MYC rearrangements/overexpression. 3. EBV Status: EBV-negative (Epstein-Barr virus-negative). |
| Immunophenotype | Strongly positive for CD19, CD20, CD22, CD23, CD37, CD38, CD40, CD5, and HLA-DR; Negative for CD3, CD10, CD11c, CD138, and cyCD3 |
| Growth Kinetics | Doubling time is approximately 30 to 45 hours; thrives best when seeded within optimal functional density ranges |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Suspension cells do not require enzymatic digestion. Please collect the entire suspension directly from the culture flask. 2. Centrifuge the cell suspension at 1000 rpm for 5 minutes. 3. Completely aspirate the spent supernatant medium, taking care not to disturb the cell pellet at the bottom. 4. Resuspend the cells in fresh, pre-warmed complete growth medium. 5. Seed the cells into a new culture flask. Please maintain a high baseline seeding density: the density should be kept at a minimum of 0.3 X 10^6 to 0.5 X 10^6 cells/mL. Do not allow the cell density to exceed 2.0 X 10^6 cells/mL. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | Split optimal suspension cultures at a ratio of 1:2 to 1:4 |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | 70% to 80% RPMI 1640 + 10% to 20% FBS + 10% DMSO |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR0484 | GFP/Luc Reporter Cell Line - Jeko-1 | Inquiry |
| CSC-RR0530 | GFP Reporter Cell Line - Jeko-1 | Inquiry |
| CSC-RR0531 | Luciferase Reporter Cell Line - Jeko-1 | Inquiry |
| CSC-RO01606 | OVAL Stable Cell Line - Jeko-1 | Inquiry |
| CSC-RO01859 | GFP/OVAL Stable Cell Line - Jeko-1 | Inquiry |
| CSC-RO02112 | Luc/OVAL Stable Cell Line - Jeko-1 | Inquiry |
| CSC-RO02365 | GFP/Luc/OVAL Stable Cell Line - Jeko-1 | Inquiry |
| CSC-RO02648 | Cas9 Stable Cell Line - JeKo-1 | Inquiry |
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