KU-812E Cell Line

Brief Introduction
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	KU-812E is a human pluripotent myeloid leukemia cell line established through specific clonal isolation from its parental cell line, KU812. The parental cell line was originally established by K. Kishi in 1985, derived from the peripheral blood of a 38-year-old Japanese male patient who was in the blast crisis phase of chronic myeloid leukemia (CML). KU-812E is globally renowned as a highly specialized pluripotent model system, exhibiting differentiation potential toward both myeloid and erythroid lineages. While retaining the fundamental characteristics of immature basophils—such as histamine synthesis capability, Fc receptor expression, and cytoplasmic metachromatic granules—the KU-812E clone is distinguished by its exceptional capacity for spontaneous and sustained terminal erythroid maturation. Upon entering the maturation phase, a significant proportion of the cells naturally differentiate into fully hemoglobinized erythroblasts. Consequently, it has become an indispensable tool worldwide for research into multilineage hematopoietic commitment, globin gene switching, and the molecular biology of basophil-erythroid progenitor cells.
Tissue	Blood / Bone Marrow; derived from peripheral blood
Disease	Chronic Myeloid Leukemia (CML) in blast crisis
Morphology	Lymphoblast-like / Lymphocyte-like
Gender	Male
Age	38 years
Product Format	Frozen
Growth Mode	Suspension
Biosafety Level	1 (Biosafety classification is based on standard international medical cell guidelines)
Applications	1. Directly dissect cell lineage commitment pathways spanning both basophil and erythroid lineages; 2. Investigate the molecular and transcriptional mechanisms underlying the synthesis of adult, fetal, and Bart's hemoglobin; 3. Elucidate the mechanisms of action of the BCR-ABL1 tyrosine kinase network and test novel targeted inhibitors; 4. Conduct preclinical evaluations of compounds that induce myeloid or erythroid differentiation; 5. Delineate the enzymatic mechanisms specific to immature human basophils (e.g., histamine synthesis and serine protease expression).
Shipped In	Dry ice
Storage Temperature	−196°C (Liquid nitrogen vapor phase)
Characteristics
Tumorigenic	Yes, retains infinite lifespan and displays baseline tumorigenic capability
Genetics / Translocation	The Philadelphia chromosome (Ph1) is positive; the karyotype harbors the characteristic t(9;22)(q34;q11) translocation, resulting in the BCR-ABL1 fusion oncogene. Furthermore, a secondary alteration—the i(17q) isochromosome—is also observed.
Erythroid Properties	Highly responsive and structurally unique compared to parental populations. Demonstrates high baseline rates of benzandine-positive mature cells (approx. 35% in cell suspension) and continuous expression of major erythroid marker proteins including glycophorins, spectrin, and band 3.
Viral Testing Markers	Evaluated by Real-time PCR and confirmed completely negative for CMV, EBV, HBV, HCV, HIV-1, HIV-2, HTLV-1, HTLV-2, BKV, and JCV.
Growth Kinetics	Proliferates cleanly in suspension with a typical doubling time of approximately 48 hours (2 days) under standard maintenance.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Since the cells grow entirely in suspension—appearing as single floating cells or loose aggregates—there is no need to use trypsin for enzymatic digestion. 2. Gently shake the culture flask to ensure the suspension is evenly distributed, then transfer the culture medium into a sterile conical centrifuge tube. 3. Centrifuge at approximately 200–300 × g (or 1000 rpm) for 5 minutes. 4. Carefully aspirate the supernatant, ensuring that the cell pellet remains undisturbed. 5. Gently resuspend the cells in pre-warmed, fresh complete culture medium. 6. Aliquot the cells into new culture vessels. Strict Density Control: Maintain the cell seeding density between approximately 1.0 × 10^5 and 2.0 × 10^5 cells/mL. Never allow the cell density to exceed the critical viability saturation point.
Medium Renewal	Every 2 to 3 days depending directly on proliferation rate and visual color shifts of the medium
Subcultivation Ratio	Maintain through simple dilution or replenishment ratios of 1:2 to 1:4
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	70% RPMI 1640 + 20% FBS + 10% DMSO

Cat.No.	Product Name	Price
CSC-RR0581	GFP Reporter Cell Line - KU-812E	Inquiry
CSC-RR0582	Luciferase Reporter Cell Line - KU-812E	Inquiry
CSC-RR0583	GFP/Luc Reporter Cell Line - KU-812E	Inquiry
CSC-RO01613	OVAL Stable Cell Line - KU-812E	Inquiry
CSC-RO01866	GFP/OVAL Stable Cell Line - KU-812E	Inquiry
CSC-RO02119	Luc/OVAL Stable Cell Line - KU-812E	Inquiry
CSC-RO02372	GFP/Luc/OVAL Stable Cell Line - KU-812E	Inquiry

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* For research use only. Not intended for any clinical use.

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