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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | MOLT-4 is an established human T-lymphoblastic cell line, originally generated by J. Minowada and colleagues in 1971. The cell line was isolated from the peripheral blood of a 19-year-old Caucasian male patient experiencing a relapse of acute lymphoblastic leukemia (ALL) following multi-drug combination chemotherapy. MOLT-4 cells exhibit a round, lymphoid morphology and grow exclusively in suspension, appearing either as single cells or as irregular clusters. The cell line is globally recognized as a fundamental and iconic in vitro model for studying T-cell leukemia and the pathogenesis of human immunodeficiency virus (HIV-1). Furthermore, MOLT-4 is a key member of the renowned NCI-60 cancer cell line panel, serving as a cornerstone in global high-throughput anti-tumor drug development and the identification of molecular targets. |
| Tissue | Blood; Peripheral Blood |
| Disease | T-cell Acute Lymphoblastic Leukemia (T-ALL) |
| Morphology | Lymphoblast-like; round cells |
| Gender | Male |
| Age | 19 years |
| Product Format | Frozen |
| Growth Mode | Suspension |
| Biosafety Level | 1 (Biosafety classification is guided by the current standard BMBL guidelines) |
| Applications | 1. A host model widely used for HIV-1/HIV-2 infection assays, studies on viral replication kinetics, and syncytium formation analysis; 2. Included in the NCI-60 screening panel for activity profiling of novel chemotherapeutic agents and targeted anti-leukemia compound libraries; 3. Used to elucidate the molecular genetic characteristics and signal transduction networks of T-cell malignancies; 4. A host target for non-viral and viral transfection studies employing specific delivery reagents; 5. Used for investigating tumorigenicity and leukemia progression in X-irradiated or untreated nude mice. |
| Shipped In | Dry ice |
| Storage Temperature | −196°C (Liquid nitrogen vapor phase only) |
| Characteristics | |
| Tumorigenic | Yes, forms tumors in untreated nude mice, anti-lymphocyte serum-treated mice, and X-irradiated mice. |
| Karyotype | Human flat-moded hypertetraploid karyotype; modal chromosome count typically shifts around 95 (ranges between 89–99). |
| Immunophenotype | T-cell markers (cyCD3, CD2, CD3, CD4, CD5, CD6, CD7, and CD8) were strongly positive; the myeloid marker CD13, the B-cell marker CD19, and TCRα/β or TCRγ/δ complexes all tested negative; and terminal deoxynucleotidyl transferase (TdT) showed high levels of endogenous expression. |
| Growth Kinetics | Rapid suspension expansion; typical population doubling time spans approximately 40 hours. Viability may transiently drop immediately after thawing recovery. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. MOLT-4 cells grow in loose suspension aggregates; enzymatic dissociation with trypsin is not required. 2. Gently agitate the culture vessel to ensure a homogeneous cell suspension, then transfer the medium to a sterile conical centrifuge tube. 3. Centrifuge at approximately 200-300 × g for 5 minutes. 4. Completely aspirate the supernatant, retaining the compact cell pellet. 5. Resuspend the cells evenly in pre-warmed, fresh complete medium. 6. Transfer to a new culture vessel. |
| Medium Renewal | Every 2 to 3 days depending on metabolic rate and acid pH visual indicators. |
| Subcultivation Ratio | Split optimal suspension cultures at a ratio of 1:2 to 1:3. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | 90% Complete growth medium + 10% DMSO (or 50% RPMI 1640 + 40% FBS + 10% DMSO) |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR0634 | Luciferase Reporter Cell Line - MOLT-4 | Inquiry |
| CSC-RO01625 | OVAL Stable Cell Line - MOLT-4 | Inquiry |
| CSC-RO01878 | GFP/OVAL Stable Cell Line - MOLT-4 | Inquiry |
| CSC-RO02131 | Luc/OVAL Stable Cell Line - MOLT-4 | Inquiry |
| CSC-RO02384 | GFP/Luc/OVAL Stable Cell Line - MOLT-4 | Inquiry |
| CSC-RO02657 | Cas9 Stable Cell Line - MOLT-4 | Inquiry |
| CSC-RR01216 | Nluc Reporter Cell Line - MOLT-4 | Inquiry |
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