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Frequently Asked Questions: Stable Cell Line


How do you generate stable cell lines?

Frequently Asked Questions: Stable Cell Line

  1. How do you generate stable cell lines?
  2. Does Creative Biogene guarantee success in making stable cell lines?
  3. What is the stability of generated stable cell lines?
  4. What are the recommended storage conditions of frozen stable cells?
  5. What is the BSL level of generated stable cell lines?

1. How do you generate stable cell lines?

Our stable cell lines are generated by either lentivirus transduction or plasmid transfection, followed by selection of stable clones.

2. Does Creative Biogene guarantee success in making stable cell lines?

We have more than 90% success rate in making stable cell lines. However, in some rare occasions, stable cells are hard to be generated due to gene toxicity, gene-host incompatibility etc.

3. What is the stability of generated stable cell lines?

It is normal for cells to experience genotypic changes. Genetic instability is a biological phenomenon that occurs in all stably transfected cells. This results in reduced responsiveness over time. So, it is critical to prepare an adequate number of frozen stocks at early passages.

4. What are the recommended storage conditions of frozen stable cells?

The cells are usually shipped frozen. After receipt, cells should be thawed and cultured immediately. If this is not possible, cells should immediately be stored in liquid nitrogen upon arrival until you are ready to thaw and propagate them.

5. What is the BSL level of generated stable cell lines?

We are unable to comment on the safety level classification because regulations vary between institutions. It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Here are the NIH guildelines for working with cells containing recombinant viral genomes for your reference: http://osp.od.nih.gov/office-biotechnology-activities/rdna/nih_guidelines_new.htm#_Section_III-E-1.

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