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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | HCC38 is a human ductal breast carcinoma cell line derived from the primary breast tumor of a 50-year-old female patient. These cells exhibit an epithelial morphology and grow as an adherent monolayer under standard culture conditions. Phenotypically, HCC38 is classified as a model of basal-like triple-negative breast cancer (TNBC), characterized by the absence of estrogen receptor (ER) and progesterone receptor (PR) expression and a lack of human epidermal growth factor receptor 2 (HER2) overexpression. Cytogenetically, the cell line displays an aneuploid karyotype with complex numerical and structural chromosomal alterations. At the molecular level, HCC38 harbors a mutation in the TP53 tumor suppressor gene. With its well-defined triple-negative phenotype and unique genomic profile, HCC38 serves as a valuable in vitro model for investigating the pathogenesis of TNBC, exploring signaling dynamics in the basal-like subtype, evaluating targeted therapies and candidate chemotherapeutic agents, and conducting high-throughput functional genomic screens. |
| Tissue | Breast |
| Disease | Primary Ductal Breast Carcinoma; Triple-Negative Breast Cancer (TNBC) |
| Morphology | Epithelial |
| Gender | Female |
| Age | 50 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Research on the biological characteristics and oncogenic mechanisms of basal-like triple-negative breast cancer (TNBC); 2. In vitro screening and preclinical evaluation of novel chemotherapy and targeted drug candidates; 3. Research on the mechanisms of breast cancer cell motility, migration, and invasion; 4. Research on functional genomics, CRISPR/Cas9 knockout screening, and target validation. |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Karyotype | Aneuploid |
| Tumorigenic | Yes, tumorigenic in immunocompromised (nude) mice |
| Genetic Profile | TP53 mutation; ER negative (ER-); PR negative (PR-); HER2 negative (HER2-) |
| Expression Markers | Positive for basal cytokeratins (CK5/6, CK14); EGFR positive |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell monolayer with PBS (without Ca2+/Mg2+) to completely remove residual serum. 3. Add 1.0 to 2.0 mL of 0.25% trypsin-0.03% EDTA solution and incubate at 37°C until the cell monolayer detaches (typically 2 to 5 minutes). 4. Add fresh complete growth medium to neutralize the trypsin and gently pipette up and down to prepare a single-cell suspension. 5. Subculture the cell suspension into new culture vessels. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is recommended |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO0217 | Cas9 Stable Cell Line - HCC38 | Inquiry |
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