HEL Cell Line

Brief Introduction
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	HEL is a human erythroleukemia cell line established in 1980 by T. Papayannopoulou and her colleagues. This cell line was isolated from the peripheral blood of a 30-year-old male patient who was experiencing a relapse of acute myeloid leukemia (classified as AML M6 / erythroleukemia) following initial treatment for Hodgkin lymphoma. In the field of hematology, HEL cells are widely recognized as the preferred model for studying erythroid differentiation and globin gene regulation. Notably, it was the first human cell line demonstrated to be capable of both spontaneous and inducible globin synthesis. Furthermore, this cell line serves internationally as a leading model system for investigating myeloproliferative neoplasms (MPNs), as it naturally harbors a homozygous JAK2 V617F mutation, which drives the constitutive activation of the JAK-STAT pathway.
Tissue	Myeloid; Peripheral Blood
Disease	Acute Myeloid Leukemia (AML FAB M6; Erythroleukemia)
Morphology	Lymphoblast-like; round, large to occasionally giant multinucleated cells
Gender	Male
Age	30 years
Product Format	Frozen
Growth Mode	Suspension (with a very small fraction of cells loosely adhering to the plastic surface)
Biosafety Level	1 (Biosafety classification is based on U.S. Public Health Service Guidelines)
Applications	1. Molecular pathology of JAK2 V617F-driven myeloproliferative neoplasms (MPNs); 2. Elucidation of the molecular mechanisms regulating hemoglobin switching and erythroid differentiation; 3. Development of models for phorbol ester (e.g., TPA/PMA)-induced macrophage-like lineage switching; 4. Preclinical drug screening of novel JAK2 kinase inhibitors and targeted anti-leukemic compounds; 5. Assessment of epigenetic and gene-silencing modifiers in erythroid malignancies.
Shipped In	Dry ice
Storage Temperature	−196°C (Liquid nitrogen vapor phase)
Characteristics
Tumorigenic	Yes, displays engraftment and leukemic development potential in immunocompromised mice
Karyotype	Human hypotriploid karyotype with approx. 2.3% polyploidy; modal chromosome distribution centered at 63 (60–64)<3n>XYY.
Genetic Profile	1. JAK2 Mutation Status: Carries the classic, homozygous, hyperactivating JAK2 V617F mutation (confirmed via RT-PCR and DNA sequencing). 2. Globin Synthesis: Natively expresses hemoglobin transcripts (specifically, including Gγ, Aγ, ε, ζ, and α globin chains).
Immunophenotype	Positive for CD13, CD33, CD34 (weakly), CD41, CD42 (weakly), CD71, and CD235a (Glycophorin A); Negative for CD3, CD14, CD15, and CD19.
Growth Kinetics	Rapid suspension expansion; doubling time is approximately 36 hours under optimal conditions.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Suspension cultures expand in the form of floating single cells and loose cell aggregates. During routine passaging, enzymatic digestion (trypsinization) is not required. 2. Uniformly collect the cell suspension from a culture vessel that is in the active growth phase, and transfer it to a sterile conical centrifuge tube. 3. Centrifuge at a relative centrifugal force of approximately 200-300 × g (or 1000 rpm) for 5 minutes. 4. Thoroughly aspirate and discard the supernatant medium, ensuring that the cell pellet remains intact. 5. Thoroughly and gently resuspend the cell pellet in pre-warmed, fresh complete growth medium. 6. Inoculate into a new culture vessel. Critical Density Window: The minimum inoculation density should be 0.5 X 10^6 cells/mL. Strictly maintain the working culture concentration between 0.2 X 10^6 and 1.0 X 10^6 cells/mL. Never exceed the maximum saturation threshold of 1.5 X 10^6 cells/mL.
Medium Renewal	Every 2 to 3 days (depending heavily on acidic pH shifts and proliferation rates)
Subcultivation Ratio	Split optimal, dense cultures at a ratio of 1:3 to 1:5 every 3 days.
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	70% Base RPMI 1640 medium + 20% FBS + 10% DMSO

Cat.No.	Product Name	Price
CSC-RO01177	Cas9 Stable Cell Line - HEL	Inquiry
CSC-RR00880	GFP Reporter Cell Line - HEL	Inquiry
CSC-RR00929	Luciferase Reporter Cell Line - HEL	Inquiry

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* For research use only. Not intended for any clinical use.

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