AR42J Cell Line

Brief Introduction
Product List

General Information
Organism	Rattus norvegicus, rat
Cell Line Description	AR42J is a conditionally responsive rat pancreatic acinar cell line derived from a transplantable exocrine pancreatic carcinoma. This cell line was originally established through chemical carcinogenesis induced by azaserine in adult Wistar rats. AR42J cells exhibit an epithelioid morphology and grow as an adherent monolayer, typically proliferating in the form of slow-growing, dense, multilayered "islands." Globally, AR42J is recognized as a preferred and indispensable in vitro model for studying pancreatic acinar cell biology in the fields of gastroenterology and oncology. It offers the unique advantage of retaining various functional characteristics of normal acinar cells, including active calcium (Ca2+) signaling cascades, receptor-mediated stimulus-secretion coupling, and the packaging of digestive enzymes. It serves as a premier research model for simulating the pathogenesis of acute pancreatitis and tracking the differentiation processes of exocrine cells.
Tissue	Pancreas; Exocrine Tissue
Cell Type	Pancreatic Acinar Cell (Amphicrine tumor proxy)
Strain	Wistar
Disease	Pancreatic Adenocarcinoma / Exocrine Neoplasia
Morphology	Epithelial-like; polygonal cells forming compact, clustered colonies
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 (Biosafety classification aligns with global standards for non-infectious rodent biomaterials)
Applications	1. Develop an in vitro "gold standard" model to study the pathophysiological mechanisms of acute pancreatitis (based on the caerulein hyperstimulation model); 2. Elucidate receptor-mediated enzyme exocytosis and stimulus-secretion coupling mechanisms; 3. Establish models for cellular transdifferentiation and the conversion of exocrine cells into endocrine or ductal lineages; 4. Investigate calcium (Ca2+) signaling networks and endoplasmic reticulum (ER) structural remodeling; 5. Conduct preclinical screening of novel therapeutic agents targeting acute pancreatic inflammation and acinar tissue injury.
Shipped In	Dry ice
Storage Temperature	−196°C (Liquid nitrogen vapor phase only)
Characteristics
Tumorigenic	Yes; reliably forms progressively expanding tumors when inoculated into athymic nude mice or syngeneic hosts.
Transformant	Chemically transformed via experimental azaserine administration in vivo.
Secretory Capability	Highly inducible digestive enzyme synthesis. Basally produces and secretes amylase, lipase, and chymotrypsinogen. Secretory activity and extensive structural maturation are dramatically enhanced following exposure to glucocorticoids.
Growth Kinetics	It is characterized by extremely slow proliferation and weak initial adhesion kinetics. Following subculture or thawing, the cells typically require 3 to 4 days to settle and attach to the surface of the culture flask before division begins.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove and discard the spent complete culture medium from the culture flask. 2. Gently rinse the cell monolayer with a standard 0.25% trypsin-0.53 mM EDTA solution to remove residual serum components containing active trypsin inhibitors. 3. Aspirate the rinse solution, add 1.0 to 2.0 mL of fresh trypsin-EDTA solution, and allow it to sit. 4. Incubate at room temperature or 37°C for 2 to 5 minutes until the cells round up and detach. Critical note: AR42J cells are susceptible to mechanical damage and irreversible clumping. Do not disturb the cells by tapping, vigorously shaking, or agitating the flask while waiting for detachment. 5. Add 6.0 to 8.0 mL of complete growth medium and resuspend the cells—breaking up any clumps—by gently pipetting up and down. 6. Transfer the cell suspension to a centrifuge tube and centrifuge at approximately 125 × g for 5 to 10 minutes. Discard the supernatant, gently resuspend the cells in fresh complete medium, and distribute them into new culture vessels.
Post-Thaw Fluid Note	Since newly thawed cells adhere slowly and grow in tightly packed, refractile, island-like clusters, complete medium replacement should be avoided during the initial days following thawing or subculture. If a medium change is necessitated by pH fluctuations, the collected supernatant should be centrifuged to recover any suspended cells, which must then be returned to the culture flask to prevent significant cell loss.
Medium Renewal	Every 3 to 4 days (or 2 times per week depending on metabolic density shifts)
Subcultivation Ratio	A low split ratio of 1:2 to 1:3 is standardly recommended due to its slow expansion kinetics.
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	60% Complete growth medium + 30% FBS + 10% DMSO

Cat.No.	Product Name	Price
CSC-RO02603	Cas9 Stable Cell Line - AR42J	Inquiry
CSC-RR00896	GFP Reporter Cell Line - AR42J	Inquiry
CSC-RR00945	Luciferase Reporter Cell Line - AR42J	Inquiry

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* For research use only. Not intended for any clinical use.

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