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| General Information | |
| Organism | Rattus norvegicus, rat |
| Cell Line Description | NRK-49F is a spontaneously immortalized rat kidney fibroblast cell line characterized by contact inhibition. This cell line originated from a specific clone derived from a normal mixed-tissue culture sample of adult rat kidney submitted by J.E. DeLarco. NRK-49F cells grow as an adherent monolayer. Because the cell line can recapitulate the fibroblast-to-myofibroblast transition upon stimulation by exogenous cytokines, it is widely used to establish models of chronic kidney disease (CKD) and renal fibrosis. Furthermore, given its non-malignant phenotype and contact inhibition, it is globally recognized as a standard tool for assaying the biological activity of transforming growth factor-alpha (TGF-α) or sarcoma growth factor (SGF). |
| Tissue | Kidney; derived from in situ renal tissue |
| Cell Type | Fibroblast |
| Breed / Strain | Osborne-Mendel |
| Disease | Normal / Non-cancerous (Spontaneously immortalized) |
| Morphology | Fibroblast-like; elongated or spindle-shaped cells |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification aligns with global laboratory guidelines for non-infectious animal materials) |
| Applications | 1. A direct in vitro model of renal interstitial fibrosis and extracellular matrix (ECM) accumulation; 2. A standard model for detecting TGF-β1-induced myofibroblast activation and α-smooth muscle actin (α-SMA) expression; 3. A targeted bioassay tool for validating transforming growth factor-alpha (TGF-α) activity; 4. Investigation of toxicological cascades, drug-induced nephrotoxicity, and ferroptosis pathways in renal biology; 5. Evaluation of cellular mechanisms regulating contact inhibition and viral/chemical transformation networks. |
| Shipped In | Dry ice |
| Storage Temperature | −196°C (Liquid nitrogen vapor phase only) |
| Characteristics | |
| Tumorigenic | No, natively non-tumorigenic under normal baseline cultivation. However, the cells are exceptionally sensitive to malignant transformation if treated with specific chemical mutagens or oncogenic viruses. |
| Growth Property Baseline | Highly contact-inhibited. Warning: Maintaining the culture at complete 100% confluence for extended periods will permanently trigger spontaneous transformation, causing cells to lose their normal contact-inhibition mechanism. |
| Expression Markers | They synthesize and express the epidermal growth factor (EGF) receptor and exhibit binding characteristics for multiplication-stimulating activity (MSA). These cells respond strongly to exogenous transforming growth factor-beta (TGF-β), upregulating fibrosis-related transcripts such as collagen and α-SMA. |
| Growth Kinetics | Moderate to rapid adherent expansion; population doubling time spans approximately 30 to 40 hours depending on passage numbers. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Completely remove and discard the spent complete culture medium. 2. Briefly rinse the cell monolayer with a 0.25% trypsin-0.03% EDTA solution to thoroughly remove residual serum components. 3. Aspirate the rinse solution, add 1.0 to 2.0 mL of fresh trypsin-EDTA solution, and incubate the culture vessel at room temperature (or 37°C) until the cells detach and become rounded. 4. Immediately add fresh culture medium to completely neutralize the enzymatic reaction. 5. Gently pipette the cell suspension up and down to ensure a uniform cell dispersion, then transfer the cells to a new culture flask. 6. Critical Confluence Warning: To prevent unwanted phenotypic changes, ensure that subculturing is performed before the cells reach full confluency (typically 70–80% density). |
| Medium Renewal | 2 times per week |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | 90% Complete culture growth medium + 10% DMSO |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO02617 | Cas9 Stable Cell Line - NRK-49F | Inquiry |
| CSC-RR00905 | GFP Reporter Cell Line - NRK-49F | Inquiry |
| CSC-RR00956 | Luciferase Reporter Cell Line - NRK-49F | Inquiry |
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