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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | REC1 is a human mantle cell lymphoma (MCL) cell line derived from the peripheral blood of a 61-year-old male patient with aggressive MCL. REC1 cells exhibit a lymphoblast-like morphology and grow primarily in suspension or as loose cell aggregates. The cell line harbors the hallmark chromosomal translocation t(11;14)(q13;q32), which juxtaposes the CCND1 gene with the immunoglobulin heavy chain locus, thereby driving the characteristic overexpression of cyclin D1. Additionally, REC1 carries mutations in key cancer-driver genes, including TP53 and ATM, reflecting the high genomic instability typical of refractory MCL. With its well-defined cytogenetic profile and stable growth characteristics, REC1 has become a valuable in vitro model for investigating MCL pathogenesis, evaluating novel targeted therapies (such as BTK inhibitors), exploring mechanisms of drug resistance, and conducting functional genomic screens. |
| Tissue | Peripheral blood / Lymph node |
| Disease | Mantle Cell Lymphoma (MCL); Non-Hodgkin Lymphoma |
| Morphology | Lymphoblast-like |
| Gender | Male |
| Age | 61 years |
| Product Format | Frozen |
| Growth Mode | Suspension |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Research on the pathogenesis of mantle cell lymphoma (MCL) and the Cyclin D1 oncogenic signaling pathway; 2. Evaluation of novel targeted therapies (including BTK inhibitors and BCL-2 inhibitors); 3. Research on cell cycle dysregulation, DNA damage repair pathways, and mechanisms of drug resistance; 4. Functional genomics, target validation, and high-throughput small-molecule screening. |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Karyotype | Aneuploid |
| Tumorigenic | Yes, forms tumors in immunocompromised mice |
| Genetic Profile | t(11;14)(q13;q32) translocation (CCND1/IGH fusion); CCND1 overexpression; TP53 mutation; ATM mutation |
| Expression Markers | Cyclin D1 positive; CD5 positive; CD19 positive; CD20 positive; FCM/surface Ig positive |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Maintain the culture by adding fresh medium or replacing the medium. 2. Establish the culture by centrifuging (1,000 rpm, 5 minutes) and resuspending the cells (viable cell density of 3 × 10⁵ to 5 × 10⁵ cells/mL). 3. Do not allow the cell concentration to exceed 1.5 × 10⁶ cells/mL. 4. Gently resuspend the cells to prepare a single-cell suspension before subculturing into a new culture vessel. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | Maintain cell density between 3 × 10⁵ and 1.5 × 10⁶ cells/mL (split ratio approximately 1:2 to 1:4) |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO02649 | Cas9 Stable Cell Line - REC1 | Inquiry |
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