KMS-12-PE Cell Line

Brief Introduction
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	KMS-12-PE is a human multiple myeloma (plasma cell myeloma) cell line established in 1987 by T. Ohtsuki and his colleagues at Kawasaki Medical School. This cell line was isolated from the pleural effusion of a 64-year-old Japanese female patient suffering from end-stage, refractory multiple myeloma (specifically, a non-immunoglobulin-secreting variant), who had previously undergone combination chemotherapy. KMS-12-PE serves as a critical biomedical resource in the field of hematologic oncology, as it naturally harbors the characteristic t(11;14)(q13;q32) translocation—a key driver of the dysregulated expression of Cyclin D1 (CCND1). Uniquely, this cell line is also concomitantly characterized by a rearrangement involving the MYC locus. Internationally, it is recognized as a preferred in vitro model, widely utilized to investigate clonal evolution, elucidate the pathophysiological mechanisms of plasma cell disorders, and evaluate the efficacy of proteasome inhibitors, immunomodulatory agents, and novel targeted therapies.
Tissue	Myeloid / Blood; derived from metastatic site (Pleural Effusion)
Disease	Multiple Myeloma / Plasma Cell Myeloma
Morphology	Lymphoblast-like / Plasma cell-like; small round cells growing singly or occasionally in loose clusters
Gender	Female
Age	64 years
Product Format	Frozen
Growth Mode	Suspension
Biosafety Level	1 (Biosafety classification is based on U.S. Public Health Service Guidelines)
Applications	1. Molecular pathology and Cyclin D1-driven oncogenic mechanisms in t(11;14)-positive multiple myeloma; 2. Elucidation of transcriptional alterations induced by concurrent IGH-CCND1 and IGH-MYC translocations; 3. Preclinical evaluation of therapeutic agents; 4. High-throughput drug screening targeting multidrug resistance in hematological malignancies; 5. Mapping of host-microenvironment interactions in plasma cell malignancies.
Shipped In	Dry ice
Storage Temperature	−196°C (Liquid nitrogen vapor phase)
Characteristics
Tumorigenic	Yes, displays engraftment potential in highly immunocompromised mouse systems (e.g., NSG mice)
Karyotype	A human hypodiploid karyotype is present, accompanied by approximately 8% polyploidy; the modal chromosome number is concentrated at 42 (range: 40–47) <2n>. It is characterized by the presence of structural rearrangements and deletions associated with chromosome loss—a hallmark feature of refractory, end-stage myeloma.
Genetic Profile	1. Oncogene Rearrangements: Drives concurrent, high-level structural rearrangements of IGH-CCND1 and IGH-MYC. 2. TP53 Mutation Status: Harbors a homozygous simple mutation: p.Arg337Leu (c.1010G>T). 3. EBV Status: EBV-negative (Epstein-Barr Virus negative).
Immunophenotype	Strongly positive for CD38, CD138, and HLA-DR; Negative for CD3, CD4, CD13, CD14, CD34, cyCD3, and cyCD68.
Growth Kinetics	Expansion rates in suspension culture range from slow to moderate, with a doubling time of approximately 60 to 80 hours. The maximum saturation density is approximately 2.5 × 10^6 cells/mL.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Suspension cultures expand as independent, floating single cells or as very small, loose cell aggregates. Enzymatic digestion is entirely unnecessary. 2. Transfer the entire cell suspension into a sterile conical centrifuge tube. 3. Centrifuge at approximately 200-300 × g (or 1000 rpm) for 5 minutes. 4. Thoroughly aspirate and discard the supernatant medium, taking care not to disturb the loose cell pellet at the bottom. 5. Gently resuspend the cells in fresh, pre-warmed complete growth medium. 6. Transfer the cells into a new culture vessel.
Medium Renewal	Every 2 to 5 days (depending heavily on cellular density shifts and pH adjustments)
Subcultivation Ratio	Split optimal suspension cultures at a ratio of 1:2 to 1:4.
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	70% to 80% RPMI 1640 medium + 10% to 20% FBS + 10% DMSO

Cat.No.	Product Name	Price
CSC-RO02612	Cas9 Stable Cell Line - KMS-12-PE	Inquiry
CSC-RR00907	GFP Reporter Cell Line - KMS-12-PE	Inquiry
CSC-RR00953	Luciferase Reporter Cell Line - KMS-12-PE	Inquiry

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* For research use only. Not intended for any clinical use.

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