SCC-25 Cell Line

Brief Introduction
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	SCC-25 is a human oral squamous cell carcinoma (OSCC) cell line established by J.G. Rheinwald and M.A. Beckett in 1981. This cell line was isolated from primary tumor tissue of the tongue in a 70-year-old male patient who had not yet commenced chemotherapy or radiotherapy at the time of tissue collection. SCC-25 cells exhibit a typical polygonal epithelial morphology and grow as an adherent monolayer. Globally renowned as one of the most widely utilized and thoroughly characterized in vitro models in the field of head and neck squamous cell carcinoma (HNSCC) research, this cell line is highly regarded. The SCC-25 cell line is extensively employed to investigate the molecular mechanisms driving oral cancer progression, the mechanisms underlying epithelial-mesenchymal transition (EMT), and the regulatory networks governing radioresistance, as well as for the preclinical screening of novel epidermal growth factor receptor (EGFR) inhibitors and combination chemotherapy regimens involving cisplatin.
Tissue	Tongue
Disease	Squamous Cell Carcinoma / Oral Cancer
Morphology	Epithelial-like; Polygonal
Gender	Male
Age	70 years
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 (Biosafety classification is based on U.S. Public Health Service Guidelines)
Applications	1. Analysis of the molecular pathology, invasion kinetics, and metastatic characteristics of Oral Squamous Cell Carcinoma (OSCC); 2. Elucidation of EGFR-mediated downstream signal transduction cascades and evaluation of targeted tyrosine kinase inhibitors; 3. Investigation into the mechanisms underlying acquired resistance to standard chemotherapeutic agents; 4. Preclinical screening of novel anti-tumor compounds and small-molecule inhibitors; 5. Establishment of orthotopic tongue or subcutaneous tumor xenograft models in immunodeficient mice.
Shipped In	Dry ice
Storage Temperature	−196°C (Liquid nitrogen vapor phase)
Characteristics
Tumorigenic	Yes, forms poorly differentiated squamous cell carcinomas when inoculated into immunocompromised (nude or SCID) mice
Karyotype	Aneuploid / Hypotetraploid; modal chromosome number centers around 72 to 78. Exhibits extensive, complex structural rearrangements and marker chromosomes common in advanced oral malignancies.
Genetic Profile	1. TP53 Status: Mutated (harboring a homozygous splice mutation/deletion, resulting in aberrant p53 expression). 2. EGFR Status: Highly expressed at both the transcript and surface protein levels (frequently serves as a target for therapeutic testing). 3. HPV Status: HPV-negative (Human Papillomavirus-negative; representative of typical tobacco/alcohol-associated Oral Squamous Cell Carcinoma [OSCC]).
Growth Kinetics	Moderate to rapid expansion; typical doubling time spans 30 to 44 hours under optimal nutritional conditions.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove and discard the spent culture medium from the culture flask. 2. Gently rinse the cell monolayer with Ca²⁺/Mg²⁺-free Dulbecco's Phosphate-Buffered Saline (DPBS) to remove residual serum components containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of 0.25% Trypsin–0.53 mM EDTA solution, ensuring the cell layer is completely submerged. 4. Incubate at 37°C for 5 to 10 minutes; observe closely under an inverted microscope until the cell layer has completely detached and the cells appear rounded. Note: SCC-25 cells may form tightly packed epithelial islands and may require 10 minutes to fully disperse. 5. Add an equal or greater volume of complete culture medium to the flask to thoroughly neutralize the trypsin activity. 6. Centrifuge at approximately 200–300 × g for 5 minutes; discard the supernatant, resuspend the cell pellet evenly in fresh medium, and aliquot into new culture vessels.
Medium Renewal	2 to 3 times per week
Subcultivation Ratio	A split ratio of 1:2 to 1:4 is standardly recommended for routine maintenance.
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	90% Complete growth medium + 10% DMSO (or 50% Basal medium + 40% FBS + 10% DMSO)

Cat.No.	Product Name	Price
CSC-RO02590	Cas9 Stable Cell Line - SCC-25	Inquiry
CSC-RR00892	GFP Reporter Cell Line - SCC-25	Inquiry
CSC-RR00941	Luciferase Reporter Cell Line - SCC-25	Inquiry

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* For research use only. Not intended for any clinical use.

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