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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | MKN7 is a human gastric adenocarcinoma cell line derived from a primary gastric cancer specimen obtained from a 39-year-old Japanese male patient. These cells exhibit an epithelial morphology, grow as an adherent monolayer in culture, and retain characteristics of well-differentiated intestinal-type gastric cancer. Cytogenetically, the cell line displays an aneuploid karyotype accompanied by various complex chromosomal alterations. At the molecular level, MKN7 cells are characterized by significant HER2 (ERBB2) gene amplification and corresponding high expression of the HER2 receptor, alongside somatic mutations in genes such as TP53. Given its prominent HER2 amplification and stable growth characteristics, MKN7 has become a valuable in vitro model for investigating gastric cancer pathogenesis, exploring HER2-driven signaling pathways, evaluating anti-HER2 targeted therapies (including monoclonal antibodies and antibody-drug conjugates), and conducting high-throughput drug screening. |
| Tissue | Stomach |
| Disease | Gastric Adenocarcinoma; Gastric Cancer (Well-differentiated) |
| Morphology | Epithelial |
| Gender | Male |
| Age | 39 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Research on the biology of well-differentiated gastric adenocarcinoma and the HER2 signaling pathway; 2. In vitro evaluation of HER2-targeted drugs, monoclonal antibodies, and antibody-drug conjugates (ADCs); 3. Research on HER2 amplification and mechanisms of drug resistance in gastric cancer; 4. High-throughput pharmacological screening and functional genomic validation. |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Karyotype | Aneuploid |
| Tumorigenic | Yes, tumorigenic in immunocompromised (nude) mice |
| Genetic Profile | HER2 (ERBB2) gene amplification/overexpression; TP53 mutation |
| Expression Markers | Positive for HER2/neu (ERBB2) and Carcinoembryonic Antigen (CEA); epithelial markers positive |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell monolayer with PBS (without Ca2+/Mg2+) to completely remove residual serum. 3. Add 1.0 to 2.0 mL of 0.25% trypsin-0.03% EDTA solution and incubate at 37°C until the cell monolayer detaches (typically 2 to 5 minutes). 4. Add fresh complete growth medium to neutralize the trypsin and gently pipette up and down to prepare a single-cell suspension. 5. Subculture the cell suspension into new culture vessels. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is recommended |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR01214 | Luciferase Reporter Cell Line - MKN7 | Inquiry |
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