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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | OC 316 is a human ovarian cancer cell line derived from the malignant ascites of a female patient diagnosed with advanced-stage ovarian serous adenocarcinoma. OC 316 cells exhibit an epithelial-like morphology and grow as an adherent monolayer under standard culture conditions. The cell line retains key biological and phenotypic characteristics of high-grade ovarian serous carcinoma, making it a valuable model for gynecologic oncology research. Cytogenetically, OC 316 displays an aneuploid karyotype characterized by complex numerical and structural chromosomal rearrangements. At the molecular level, the cell line harbors genetic alterations in key tumor suppressor genes and oncogenic pathways (such as TP53 mutations) commonly implicated in the pathogenesis of ovarian cancer. Given its well-defined malignant origin and reliable in vitro growth characteristics, OC 316 has become an important research model for investigating ovarian cancer biology, studying mechanisms of platinum resistance, evaluating targeted therapies, and conducting functional genomic screens. |
| Tissue | Ovary; derived from metastatic site (Ascites) |
| Disease | Ovarian Serous Adenocarcinoma; Ovarian Cancer |
| Morphology | Epithelial |
| Gender | Female |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Research on the biological characteristics of high-grade serous ovarian cancer (HGSOC) and the tumor microenvironment of ascites; 2. Evaluation and screening of novel chemotherapeutic agents, platinum-based regimens, and candidate targeted therapies; 3. Research on mechanisms of chemotherapy resistance, DNA damage repair pathways, and cell motility in ovarian cancer; 4. Functional genomic analysis, identification of drug targets, and validation via CRISPR/Cas9 screening. |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Karyotype | Aneuploid |
| Tumorigenic | Yes, forms tumors in immunocompromised mice |
| Genetic Profile | TP53 mutation; ovarian cancer-associated driver alterations |
| Expression Markers | Positive for epithelial markers (Cytokeratins); CA125 / MUC16 positive |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell monolayer with PBS (without Ca2+/Mg2+) to completely remove residual serum. 3. Add 1.0 to 2.0 mL of 0.25% trypsin-0.03% EDTA solution and incubate at 37°C until the cell monolayer detaches (typically 2 to 5 minutes). 4. Add fresh complete growth medium to neutralize the trypsin and gently pipette up and down to prepare a single-cell suspension. 5. Subculture the cell suspension into new culture vessels. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is recommended |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR0348 | GFP Reporter Cell Line - OC 316 | Inquiry |
| CSC-RO01533 | OVAL Stable Cell Line - OC 316 | Inquiry |
| CSC-RO01786 | GFP/OVAL Stable Cell Line - OC 316 | Inquiry |
| CSC-RO02039 | Luc/OVAL Stable Cell Line - OC 316 | Inquiry |
| CSC-RO02292 | GFP/Luc/OVAL Stable Cell Line - OC 316 | Inquiry |
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