SN12C Cell Line

Brief Introduction
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	SN12C is a human renal cell carcinoma (RCC) cell line established in 1986 by Seiji Naito, Andrew C. von Eschenbach, and Isaiah Joshua Fidler at the University of Texas MD Anderson Cancer Center. The cell line was derived from a primary surgical renal tumor specimen obtained from a 43-year-old Caucasian male patient with RCC. SN12C cells exhibit an epithelial-like morphology and grow as an adherent monolayer. As a member of the well-known NCI-60 panel of cancer cell lines, SN12C serves as a widely used preclinical model for studying RCC metastasis and the tumor microenvironment. The cell line is noted for its distinct organ-specific metastatic properties: when orthotopically implanted under the renal capsule of nude mice, the cells demonstrate high tumorigenicity and consistently metastasize to the lungs, liver, and regional lymph nodes, making them a key model for investigating the mechanical mechanisms of tumor colonization.
Tissue	Kidney
Cell Type	Epithelial cell
Population / Ancestry	Caucasian
Disease	Renal Cell Carcinoma (RCC)
Morphology	Epithelial-like
Gender	Male
Age	43 years
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 (Biosafety classification matches standard laboratory parameters for non-infectious human tumor cells)
Applications	1. NCI-60 cell line core models for the high-throughput screening of novel chemotherapeutic agents and small-molecule targeted drug candidates; 2. Key in vitro and in vivo models for studying renal cancer invasion, migration, and organ-specific orthotopic metastasis; 3. Elucidation of the molecular roles of growth factors and cytokines (e.g., VEGF, HGF, and IL-8) in driving angiogenesis and tumor angiogenic signaling; 4. Investigation of mechanisms underlying chemotherapy resistance and the inhibition of targeted pathways (e.g., the PI3K/AKT/mTOR pathway) in renal cell carcinoma (RCC); 5. Assessment of cell adhesion molecules, integrin expression profiles, and protease secretion during basement membrane degradation.
Shipped In	Dry ice
Storage Temperature	−196°C
Characteristics
Tumorigenic	Yes, highly tumorigenic and metastatic; forms expanding, invasive tumors when inoculated orthotopically (under the renal capsule) or subcutaneously into athymic nude mice.
Transformant	None; immortalized spontaneously from advanced primary renal carcinoma tissue.
Growth Kinetics	Robust and rapid adherent proliferation; typical population doubling time is approximately 25.9 to 29.5 hours.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove and discard all used complete culture medium from the culture dish. 2. Gently rinse the cell monolayer with sterile Ca2+/Mg2+-free PBS to remove any residual traces of serum containing active trypsin inhibitors. 3. Add 1.0 to 2.5 mL of pre-warmed 0.25% trypsin-0.53 mM (0.03%) EDTA solution, ensuring the cell layer is completely covered. 4. Incubate at 37°C for 2 to 5 minutes; monitor closely under an inverted microscope until the cells round up, loosen, and detach completely. 5. Immediately add 8.0 to 10.0 mL of complete culture medium to neutralize trypsin activity. 6. Gently pipette the cell suspension up and down to disperse the cells into a uniform single-cell suspension. 7. Centrifuge at approximately 300 × g for 5 minutes, carefully aspirate the supernatant, resuspend the cell pellet uniformly in fresh complete growth medium, and distribute the suspension into new vessels.
Thawing Protocol Note	SN12C cells are sensitive to drastic pH fluctuations during the early stages of recovery from cryopreservation. Before adding the thawed cell suspension, the culture vessel containing fresh complete growth medium should be placed in a 5% CO2 incubator for at least 15 minutes to allow the medium to reach and equilibrate within the normal physiological pH range (7.0 to 7.6).
Medium Renewal	2 to 3 times per week
Subcultivation Ratio	Split confluent cultures (70–80% density) at a standard ratio of 1:3 to 1:8 depending on experimental scheduling.
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	90% Complete culture growth medium + 10% DMSO (or 50% Basal medium + 40% FBS + 10% DMSO)

Cat.No.	Product Name	Price
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* For research use only. Not intended for any clinical use.

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