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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | SNU-398 was established by Park et al. in 1990. This cell line was derived from primary hepatocellular carcinoma (HCC) tissue obtained from a 42-year-old Korean male patient. The patient had previously undergone treatment for a Hepatitis B virus (HBV) infection. SNU-398 cells exhibit an epithelial-like morphology and have been confirmed to harbor integrated HBV DNA sequences. Characterized by high motility and invasiveness, this cell line frequently serves as a representative model for liver cancer studies featuring mesenchymal characteristics (EMT); it is widely utilized to investigate the regulation of liver cancer cell growth, signal transduction pathways, HBV-mediated oncogenic mechanisms, and the evaluation of the efficacy of anticancer drugs. |
| Tissue | Liver |
| Disease | Hepatocellular Carcinoma (HCC) |
| Morphology | Epithelial-like; grows as an adherent monolayer |
| Gender | Male |
| Age | 42 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 2 (Due to the presence of HBV DNA sequences) |
| Applications | 1. Investigation into the pathogenesis of hepatocellular carcinoma (HCC) and tumor cell signal transduction 2. Study on the impact of hepatitis B virus (HBV) integration on the malignant transformation of hepatocytes 3. Investigation into the mechanisms of epithelial-mesenchymal transition (EMT), tumor migration, and invasion 4. In vitro screening of targeted agents and chemotherapeutic drugs for hepatocellular carcinoma 5. Establishment of human hepatocellular carcinoma xenograft mouse models |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, in immunocompromised (nude) mice |
| Karyotype | Aneuploid; modal number = 77; complex chromosomal rearrangements are present. |
| Genetic Profile | 1. HBV Status: Carries integrated HBV DNA sequences (no viral production) 2. Mutations: TP53 mutation (p.S215I); CTNNB1 (β-catenin) Wild-type |
| Growth Kinetics | The doubling time is approximately 24–32 hours. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Discard the old culture medium. 2. Wash the cell monolayer with Ca²⁺/Mg²⁺-free PBS. 3. Add 0.25% Trypsin – 0.53 mM EDTA and incubate at 37°C for approximately 5–10 minutes until the cells detach (as these cells adhere firmly, a longer incubation time may be required). 4. Add serum-containing complete medium to neutralize the enzymatic digestion, then gently pipette to mix thoroughly. 5. Seed the cells into new culture vessels at the appropriate ratio. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | 1:3 to 1:6 |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | 90% Complete growth medium + 10% DMSO |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO02620 | Cas9 Stable Cell Line - SNU398 | Inquiry |
| CSC-RR00911 | GFP Reporter Cell Line - SNU398 | Inquiry |
| CSC-RR00971 | Luciferase Reporter Cell Line - SNU398 | Inquiry |
| CSC-RR00994 | GFP/Luc Reporter Cell Line - SNU-398 | Inquiry |
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