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Frequently Asked Questions: siRNA Libraries


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Frequently Asked Questions: siRNA Libraries

  1. What is siRNA?
  2. What are the types of siRNA libraries?
  3. What about the application of siRNA library?
  4. What is the siRNA library format?
  5. What are the advantages of your siRNA library?

1. What is siRNA?

RNA interference (RNAi) is a process of gene expression silencing induced by double-stranded RNA (dsRNA). It has become extensively used in the functional study of genes immediately after the discovery that small interfering RNA (siRNA) was the intermediate effector molecule of the RNAi process. siRNA are about 19-23 bp short dsRNA molecules, which upon entering cells, will be incorporated into a protein complex called RNA induced gene silence complex (RISC). Then, one of the two strands of RNA is destructed and the other strand will remain in the RISC complex as a guide sequence for target mRNA recognition and cleavage.

2. What are the types of siRNA libraries?

Generally, there are three types of siRNA libraries: siRNA chemically synthesized, siRNA formed through enzymatic reactions and siRNA encoded by vectors.
siRNA libraries formed by chemical synthesis are regarded to be better defined in composition and easier to use, but inherently more expensive material-wise. Moreover, the designed siRNA can be directly fed into the high throughput RNA synthesis system.

3. What about the application of siRNA library?

Library screening using genome-wide siRNAs as well as miRNAs allows researchers to analyze an entire signaling pathway of tens of thousands of genes. Creative Biogene has developed siRNA libraries composed of large numbers of siRNAs which are used to identify sets of genes functionally involved in a particular biological process or disease metabolism. siRNA library screening is now widely used by pharma companies for validation of drug targets.

4. What is the siRNA library format?

Library plates are received in 96 or 384 well formats. These plates are resuspended and aliquoted into working stock library plates. These plates are made available in both 96 and 384 well formats at concentrations appropriate for screening.

5. What are the advantages of your siRNA library?

Our predesigned siRNA and siRNA libraries were designed to reduce off-target effects and provide guaranteed gene silencing. The advantages of our siRNA library including:

  • The most complete solution for reducing off-target effects up to 90%.
  • Cutting-edge bioinformatics applies seed-region analysis to reduce miRNA-like off-target effects.
  • Delivery of high confidence RNAi results available through combining guaranteed silencing with unmatched strategies for greater target specificity.
  • Pooling reduces the concentration of each individual siRNA helping to minimize off-target effects and toxicity.

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