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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | FaDu is a human epithelial cell line established by S.R.R. Rangan in 1968. This cell line was derived from a punch biopsy of a primary hypopharyngeal carcinoma in situ excised from a 56-year-old male patient of Indian/Hindu background. The patient was diagnosed with hypopharyngeal squamous cell carcinoma (HNSCC); in its native state, the tumor specimen retained characteristic bundles of tonofilaments and desmosomal regions. Globally, FaDu is recognized as a preferred foundational in vitro model system for the study of head and neck cancers. Given its status as an HPV-negative cell line, it serves as a critical representative model for simulating the oncogenic pathways typical of squamous cell carcinomas that are associated with tobacco and alcohol consumption. Currently, this cell line is widely utilized to investigate mechanisms of non-viral-induced oral tumorigenesis, evaluate drug delivery systems, assess cellular responses to radiation, and conduct high-throughput screening experiments in the fields of cancer biology and tumor immunology. |
| Tissue | Pharynx; Hypopharynx |
| Disease | Hypopharyngeal Squamous Cell Carcinoma (HNSCC) |
| Morphology | Epithelial; polygonal adherent cells growing in cohesive blocks |
| Gender | Male |
| Age | 56 years |
| Product Format | Frozen |
| Growth Mode | Adherent Monolayer |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Construction of direct transfection host systems and molecular modeling for head and neck oncology; 2. Elucidation of mechanisms of radioresistance and assessment of DNA damage repair pathways; 3. Efficacy testing of small molecules, targeted chemotherapeutic agents, and biologics; 4. Establishment of highly reliable subcutaneous or orthotopic squamous cell xenograft models in nude mice; 5. Investigation of targeted drug delivery kinetics and mechanisms of intracellular protein tracking. |
| Shipped In | Dry ice |
| Storage Temperature | −196°C (Liquid nitrogen vapor phase only) |
| Characteristics | |
| Tumorigenic | Yes, reliably forms well-differentiated squamous cell carcinomas (Grade I epidermoid carcinoma) when engrafted into nude mice. |
| Karyotype | Human hypodiploid to hypertriploid karyotype; the modal chromosome number centers around 64. It exhibits complex structural microdeletions and numerical abnormalities. |
| Genetic Profile | 1. TP53 Status: Mutated (harbors a non-rearranged splice mutation resulting in p53 processing dysfunction). 2. HPV Status: HPV-negative (Human Papillomavirus-negative). 3. Microsatellite Status: Stable (MSS). 4. Amplification: Naturally harbors amplified levels of EMS-1 (Cortactin) transcripts. |
| Isoenzymes | AK-1 (1); ES-D (1); G6PD (B); GLO-I (2); Me-2 (2); PGM1 (2); PGM3 (1). |
| Growth Kinetics | Highly proliferative; doubling time spans 30 to 50 hours depending on subculture numbers and nutritional status. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Completely remove and discard the waste medium from the culture flask. 2. Rapidly rinse the cell monolayer with 0.25% Trypsin–0.53 mM (0.03%) EDTA solution to remove residual serum containing active protease inhibitors. 3. Add 1.0 to 2.0 mL of Trypsin–EDTA solution, ensuring the cell layer is completely submerged. 4. Allow the culture flask to stand at room temperature (or at 37°C to accelerate the reaction) until the cells visibly detach from the substrate and round up. Note: Avoid mechanical disturbances—such as tapping or shaking the flask—to prevent the cells from clumping together. 5. Add an equal volume of complete culture medium to the flask, and gently pipette the mixture up and down to ensure the cell suspension is evenly distributed. 6. Centrifuge the suspension at approximately 125 × g for 5 to 10 minutes; aspirate the supernatant, gently resuspend the cell pellet in fresh complete culture medium, and proceed with seeding. |
| Thawing Protocol Note | FaDu cells are extremely sensitive to the alkalinity of the culture medium during the initial recovery phase. It is strongly recommended that, prior to cell seeding, the culture flask containing complete medium be placed in the incubator for at least 15 minutes to allow it to fully equilibrate to the physiologically appropriate range (pH 7.0 to 7.6). |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | Split optimal, confluent cultures at a ratio of 1:3 to 1:6. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | 90% Complete growth medium + 10% DMSO (or 50% EMEM + 40% FBS + 10% DMSO) |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR0146 | GFP Stable Cell Line-FaDu | Inquiry |
| CSC-RR0147 | RFP Stable Cell Line-FaDu | Inquiry |
| CSC-RR00760 | Luciferase Reporter Cell Line - FAdu | Inquiry |
| CSC-RO01469 | OVAL Stable Cell Line - FaDu | Inquiry |
| CSC-RO01722 | GFP/OVAL Stable Cell Line - FaDu | Inquiry |
| CSC-RO01975 | Luc/OVAL Stable Cell Line - FaDu | Inquiry |
| CSC-RO02228 | GFP/Luc/OVAL Stable Cell Line - FaDu | Inquiry |
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