Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | SK-MEL-30 is a human melanoma cell line derived from a subcutaneous metastasis in a 67-year-old male patient with malignant melanoma. The cell line exhibits a mixed morphology of epithelioid and spindle-shaped cells and grows as an adherent monolayer in culture. Genetically, it displays an aneuploid karyotype characterized by numerical and structural chromosomal abnormalities. Molecular profiling reveals that SK-MEL-30 harbors the classic NRAS mutation (Q61K) and expresses wild-type BRAF, making it a representative in vitro model for studying NRAS-mutant melanoma. Furthermore, the cell line retains key melanoma markers, including S100 and tyrosinase. With its well-defined genetic profile and robust proliferative capacity, SK-MEL-30 serves as a vital biological platform for investigating melanoma pathogenesis, exploring signaling dynamics within the NRAS/MEK/ERK pathway, evaluating novel targeted therapies, and conducting high-throughput drug screening. |
| Tissue | Skin; derived from metastatic site (Subcutaneous tissue) |
| Disease | Malignant Melanoma |
| Morphology | Epithelial-like / Spindle-shaped |
| Gender | Male |
| Age | 67 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Research on the biological characteristics and metastatic mechanisms of NRAS-mutant malignant melanoma; 2. Evaluation and screening of targeted therapies (particularly MEK and ERK pathway inhibitors); 3. Research on melanoma immunogenicity, melanogenesis, and resistance to MAPK pathway inhibition; 4. Functional genomic analysis, target identification, and CRISPR/Cas9 knockout screening. |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Karyotype | Aneuploid |
| Tumorigenic | Yes, tumorigenic in immunocompromised (nude) mice |
| Genetic Profile | NRAS mutation (Q61K); BRAF wild-type; TP53 mutation |
| Expression Markers | S100 protein positive; Tyrosinase positive; Melan-A (MART-1) positive |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell monolayer with PBS (without Ca2+/Mg2+) to completely remove residual serum. 3. Add 1.0 to 2.0 mL of 0.25% trypsin-0.03% EDTA solution and incubate at 37°C until the cell monolayer detaches (typically 2 to 5 minutes). 4. Add fresh complete growth medium to neutralize the trypsin and gently pipette up and down to prepare a single-cell suspension. 5. Subculture the cell suspension into new culture vessels. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is recommended |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR0353 | GFP Reporter Cell Line - SK-MEL-30 | Inquiry |
| CSC-RO01561 | OVAL Stable Cell Line - SK-MEL-30 | Inquiry |
| CSC-RO01814 | GFP/OVAL Stable Cell Line - SK-MEL-30 | Inquiry |
| CSC-RO02067 | Luc/OVAL Stable Cell Line - SK-MEL-30 | Inquiry |
| CSC-RO02320 | GFP/Luc/OVAL Stable Cell Line - SK-MEL-30 | Inquiry |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
Copyright © 2026 Creative Biogene. All rights reserved.